摘要： 通过研究油菜体内过氧化物酶(POD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)含量及活性变化规律与黑胫病侵染过程、品种抗病性的关系,揭示不同油菜品种对黑胫病菌的抗病机制。用紫外分光光度计测定并比较分析抗、感品种接种前后,各种防御酶活性的变化规律。接种前,抗病品种的POD、PPO、CAT活性高于感病品种,PAL、SOD活性则与感病品种相近。接种后抗病品种表现出较高的敏感性,5种防御酶活性均迅速升高而达到峰值,且其酶活性增加量显著大于感病品种;而感病品种防御酶的活性或增幅较小或较抗病品种滞后。黑胫病菌可以诱导油菜体内5种防御酶活性变化,POD、PPO、CAT、PAL、SOD的酶活性与油菜的抗病性具有正相关关系,这些防御酶活性峰值出现的高低与早晚,可作为早期鉴定油菜抗黑胫病的生理指标。

关键词: 油菜, 防御酶, 黑胫病菌

Abstract: In order to explore the resistance mechanism of different rapeseed varieties to Leptosphaeria biglobosa, we analyzed the relation between the changes in contents and activities of defense enzymes peroxidase(POD), polyphemol oxidase(PPO), catalase (CAT), phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) in different rapeseed cultivars with the infection process and varieties resistance.The activities of POD, PPO, CAT, PAL and SOD in resistant and susceptible rapeseed varieties before or after infected were measured using spectrophotometer.For uninfected plant, the resistance varieties had a higher activities of POD, PPO, CAT than susceptible varieties and had an almost the same values of PAL, SOD.After inoculation, resistant varieties showed higher sensitivity to Leptosphaeria biglobosa, the activities of five defense enzyme increased rapidly and reached the peak, the increment were significantly greater than susceptible materials.The enzyme activities of susceptible materials rise more slowly or later than resistant varieties. Leptosphaeria biglobosa can induced the changes of five defense enzymes in rapeseed, the enzyme activities of POD, PPO, CAT, PAL and SOD had a positive correlation with rapeseed resistance.These results suggested that early rapid increase of enzyme activities might be used as one of physiological indices for selection of rapeseed resistant varieties to L.biglobosa.

Key words: Rapeseed, Defense enzymes, Leptosphaeria biglobosa

中图分类号: 

• S432.2+2