华北农学报 ›› 2015, Vol. 30 ›› Issue (2): 104-109. doi: 10.7668/hbnxb.2015.02.019

所属专题: 甜椒辣椒

• 论文 • 上一篇    下一篇

不同地区辣椒疫霉菌生理小种的鉴定和生物学特性的研究

杨叔青1,2, 胡栓红2, 杨志刚2, 陈聪1, 徐晓美3, 高婧1, 王勇2, 赵君1   

  1. 1. 内蒙古农业大学 农学院, 内蒙古 呼和浩特 010019;
    2. 内蒙古农牧业科学院 蔬菜研究所, 内蒙古 呼和浩特 010031;
    3. 广东省农业科学院 蔬菜研究所, 广东 广州 510640
  • 收稿日期:2015-02-04 出版日期:2015-04-28
  • 通讯作者: 赵君(1969-),女,内蒙古呼和浩特人,教授,博士,博士生导师,主要从事分子植物病理学研究。
  • 作者简介:杨叔青(1986-),女,内蒙古土默特左旗人,在读博士,主要从事分子植物病理学研究。
  • 基金资助:
    国家自然科学基金项目(31260425);内蒙古科技厅应用与研究开发计划项目(20110711);内蒙古农牧业科技创新基金项目(2015CXJJNo3)

The Study on the Physiological Race Type and Biological Characteristics of Phytophthora capsici Collected from Different Regions

YANG Shu-qing1,2, HU Shuan-hong2, YANG Zhi-gang2, CHEN Cong1, XU Xiao-mei3, GAO Jing1, WANG Yong2, ZHAO Jun1   

  1. 1. Agricultural College, Inner Mongolia Agricultural University, Huhhot 010019, China;
    2. Vegetable Institute, Inner Mongolia Academy of Agricultural and Animal Sciences, Huhhot 010031, China;
    3. Vegetable Research Institute, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, China
  • Received:2015-02-04 Published:2015-04-28

摘要: 为了确定不同地区采集的辣椒疫病病原菌的菌种类型及其生理小种类型,首先利用形态学和分子生物学的手段对广东、山西和内蒙古等地的辣椒疫病的病原菌进行了鉴定,结果表明,所分离到的7株病原菌均为辣椒疫霉菌。通过比较不同地区分离到的疫霉菌生物学特性,发现不同地区辣椒疫霉菌在菌落形态、孢子囊的数量、生长速度等方面存在显著的差异。利用国际通用的鉴别寄主,对分离到的7个辣椒疫霉菌进行生理小种的鉴定,结果表明,来源于广东的辣椒疫霉菌P1是2号生理小种,而来源于山西和内蒙古的6株辣椒疫霉菌P2~P7都是3号生理小种。目前,在山西和内蒙古地区并没有关于辣椒疫霉菌生理小种鉴定的任何报道,确定了这2个地区的辣椒疫霉菌的优势生理小种为3号生理小种。

关键词: 辣椒疫病, 辣椒疫霉菌, 生理小种, 生物学特性

Abstract: In order to confirm the strain type and physiological race type of the pathogen causing Pepper blight and being collected from different regions, we utilized the morphological and molecular methods to identify the seven strains which were collected from Guangdong, Shanxi and Inner Mongolia as Phytophthora capsici Leonian.Through the comparison of biological characteristics of the seven strains from different regions, we found there were significant differences in colonial morphology, mycelial growth rate and sporulation ability.Through a set of the international differential hosts, we identified physiological race type of the seven strains, P1 which was from Guangdong belonged to Race 2, P2 to P7 which were collected from Shanxi and Inner Mongolia all belonged to Race 3.At present, there is no report of the physiological race type of P.capsici in Shanxi and Inner Mongolia, our research's results confirmed that the physiological race type of P.capsici in these two regions is Race 3.This results not only filled the vacancy in the research field of P.capsici, it would also lay a solid foundation for the resistance breeding against Pepper blight in the future.

Key words: Pepper blight, Phytophthora capsici Leonian, Physiological race type, Biological characteristics

中图分类号: 

引用本文

杨叔青, 胡栓红, 杨志刚, 陈聪, 徐晓美, 高婧, 王勇, 赵君. 不同地区辣椒疫霉菌生理小种的鉴定和生物学特性的研究[J]. 华北农学报, 2015, 30(2): 104-109. doi: 10.7668/hbnxb.2015.02.019.

YANG Shu-qing, HU Shuan-hong, YANG Zhi-gang, CHEN Cong, XU Xiao-mei, GAO Jing, WANG Yong, ZHAO Jun. The Study on the Physiological Race Type and Biological Characteristics of Phytophthora capsici Collected from Different Regions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 104-109. doi: 10.7668/hbnxb.2015.02.019.

使用本文

0
    /   /   推荐 /   导出引用

链接本文: http://www.hbnxb.net/CN/10.7668/hbnxb.2015.02.019

               http://www.hbnxb.net/CN/Y2015/V30/I2/104