副猪嗜血杆菌<em>vacJ</em>基因缺失菌株构建及其生物学特性分析

doi:10.7668/hbnxb.2017.05.004

华北农学报 ›› 2017, Vol. 32 ›› Issue (5): 19-24. doi: 10.7668/hbnxb.2017.05.004

所属专题： 畜牧；生物技术；

副猪嗜血杆菌vacJ基因缺失菌株构建及其生物学特性分析

赵良友^1,2, 高雪丽¹, 刘超男¹, 申海龙⁴, 姜南³, 万伟泉¹, 吕晓萍¹, 郑世民¹

1. 东北农业大学动物医学学院, 黑龙江省实验动物与比较医学重点实验室, 黑龙江哈尔滨 150030;
2. 黑龙江中医药大学, 药物安全性评价中心, 黑龙江哈尔滨 150040;
3. 大连大学生命科学与技术学院, 辽宁大连 116622;
4. 浙江省湖州市长兴县农业局, 浙江湖州 313100

收稿日期:2017-08-03 出版日期:2017-10-28
通讯作者: 郑世民(1959-),男,黑龙江哈尔滨人,教授,博士,主要从事动物免疫病理研究。
作者简介:赵良友(1982-),男,黑龙江哈尔滨人,助理研究员,在读博士,主要从事动物免疫病理和毒性病理研究。
基金资助:
国家自然科学基金项目（31472169）

Construction and Biological Characterization of vacJ Gene Deletion Strain of Haemophilus parasuis

ZHAO Liangyou^1,2, GAO Xueli¹, LIU Chaonan¹, SHEN Hailong⁴, JIANG Nan³, WAN Weiquan¹, LÜ Xiaoping¹, ZHENG Shimin¹

1. College of Veterinary Medicine, Northeast Agricultural University, Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin 150030, China;
2. Drug Safety Evaluation Center of Heilongjiang University of Chinese Medicine, Harbin 150040, China;
3. College of Life Science and Technology, Dalian University, Dalian 116622, China;
4. Agriculture Bureau of Changxing, Huzhou 313100, China

Received:2017-08-03 Published:2017-10-28

摘要/Abstract

摘要： 为了探究副猪嗜血杆菌外膜脂蛋白编码基因vacJ在其致病中的作用，以血清5型临诊分离株HS49为研究对象，构建了vacJ基因缺失菌株（△ vacJ），进而比较了野生株与缺失株在生长特性、对细胞的黏附及入侵和毒力等方面的差异。结果显示，与野生株相比较，缺失vacJ后的菌株生长速率明显下降，对PK-15细胞的黏附和入侵能力则明显降低，形成生物被膜的能力也显著下降。缺失vacJ菌株对Balb/C小鼠感染模型的LD₅₀是野生株的14倍，表明vacJ基因敲除后，副猪嗜血杆菌毒力明显下降。表明vacJ 基因与副猪嗜血杆菌生长、黏附入侵、生物被膜形成及毒力密切相关。

关键词: 副猪嗜血杆菌, vacJ基因, 生物学特性, 毒力

Abstract: To investigate the role of the vacJ gene in Haemophilus parasuis (HPS) virulent strain HS49,the vacJ gene-deletion mutant △ vacJ strain was constructed. To better assess the role of vacJ gene in the virulence of HPS,growth characteristics,adhesion and invasion to host cells,and experimental infection of mice were performed. The results supported that compared to the wild-type strain,the growth rate of vacJ deletion strain was decreased. The △ vacJ deletion strain exhibited a significantly decrease ability to adhere to and invade PK-15 cell. Furthermore,the △ vacJ was attenuated in a murine (Balb/C) model of infection and its LD₅₀ value was approximately fourteen-fold higher than the wild-type. The data obtained in this study indicate that vacJ plays an essential role in growth,adherence to and invasion of host cells,biofilm formation,and related to HPS and further suggest a putative role of vacJ lipoprotein in virulence regulation.

Key words: Haemophilus parasuis, vacJ gene, Biological characteristics, Virulence

中图分类号:

赵良友, 高雪丽, 刘超男, 申海龙, 姜南, 万伟泉, 吕晓萍, 郑世民. 副猪嗜血杆菌vacJ基因缺失菌株构建及其生物学特性分析[J]. 华北农学报, 2017, 32(5): 19-24. doi: 10.7668/hbnxb.2017.05.004.

ZHAO Liangyou, GAO Xueli, LIU Chaonan, SHEN Hailong, JIANG Nan, WAN Weiquan, LÜ Xiaoping, ZHENG Shimin. Construction and Biological Characterization of vacJ Gene Deletion Strain of Haemophilus parasuis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 19-24. doi: 10.7668/hbnxb.2017.05.004.

http://www.hbnxb.net/CN/Y2017/V32/I5/19

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副猪嗜血杆菌vacJ基因缺失菌株构建及其生物学特性分析

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