不同抗蚜性小麦品种(系)的遗传多样性SSR标记分析

doi:10.7668/hbnxb.2014.05.020

摘要/Abstract

摘要： 为了揭示小麦抗蚜品种(系)的遗传多样性,在田间蚜虫圃对150份小麦品种(系)孕穗期和灌浆期蚜量比值进行2年对比的基础上,选取抗性结果较为一致的材料,利用筛选的54对具有多态性的SSR标记检测了43个不同抗蚜水平小麦品种(系)的遗传多态性。结果表明,54对SSR标记在这43份不同抗蚜水平小麦品种(系)中检测到365个等位变异,每个引物检测到2~18个等位变异,平均为6.7个;从每条谱带在所有材料中出现的频率来看,变异范围为2.3%~97.7%;多态性信息量为0.05~0.91,平均为0.65;43个小麦品种间的相对遗传距离为0.30~0.90,平均为0.52;SSR标记聚类分析在相对遗传距离为0.55处将43个不同抗蚜水平小麦品种(系)分为五大类群。选育和推广抗虫品种时尽可能选择聚类图中亲缘关系较远的材料;抗蚜品种京冬6号单独聚为一类,与其余品种亲缘关系较远,可作为新的抗源用于抗蚜育种。因此,用SSR分子标记分析不同抗蚜水平小麦品种(系)间的遗传多样性和亲缘关系,可以为培育和推广抗蚜小麦品种提供参考。

关键词: 小麦, 麦蚜, SSR, 遗传多样性

Abstract: In order to explore the genetic diversity of wheat varieties with different aphid-resistant degrees,and then offer the reference for breeding new aphid-resistant cultivars,43 wheat varieties/lines with different aphid-resistant degrees,based on a two-year field appraisal results for aphid index by nature aphid populations,were studied by Simple sequence repeat(SSR)markers.The results showed that fifty-four markers were screened and a total of 365 allelic variants were detected in these 43 varieties/lines,ranging from 2 to 18 alleles per marker with an average of 6.7. The PIC(Polymorphism information contents)for each polymorphic primer varied from 0.05-0.91,with an average of 0.65.The mean genetic distance(GD)among 43 varieties was 0.52,varying from 0.30-0.90.Based on the 0.55 of GD 43 varieties were clustered into five groups.Resistant insect variety Jingdong 6 was a group by oneself.There was a further genetic relationship between Jingdong 6 and other varieties,and so the Jingdong 6 might be used as a resistant insect parent.Therefore,SSR analysis could reliable determine genetic relationships within a diverse array of wheat varieties and effectively provide the data for selecting parents for breeding programme.

Key words: Wheat, Aphid, Simple sequence repeats(SSR), Genetic diversity

中图分类号:

许兰杰, 侯起岭, 侯春雨, 张婷, 张小华, 倪中福, 梁荣奇. 不同抗蚜性小麦品种(系)的遗传多样性SSR标记分析[J]. 华北农学报, 2014, 29(5): 119-124. doi: 10.7668/hbnxb.2014.05.020.

XU Lan-jie, HOU Qi-ling, HOU Chun-yu, ZHANG Ting, ZHANG Xiao-hua, NI Zhong-fu, LIANG Rong-qi. Genetic Diversity Analysis of Wheat Varieties with Different Aphids-resistant by Simple Sequence Repeat(SSR) Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 119-124. doi: 10.7668/hbnxb.2014.05.020.

http://www.hbnxb.net/CN/Y2014/V29/I5/119

参考文献

[1] 李贤庆,郭线茹,李克斌,等.不同小麦品种(系)对麦长管蚜的抗性[J].昆虫学报,2006,49(6):963-968.
[2] 徐利敏,齐凤鸣,张建平,等.麦长管蚜危害小麦产量损失的初步研究[J].内蒙古农业科技,1998(5):28,41.
[3] 张乃芹,侯传本,于凌春,等.麦田主要天敌昆虫种群动态及对麦蚜控制效应的研究[J].山东农业科学,2007(5):86-88.
[4] 魏岑,苑贤林,孙小平,等.张掖麦蚜对菊酯类杀虫剂的抗药性[J].昆虫学报,1990,33(1):117-120.
[5] 李凤珍,吉万全,吴金华.小麦抗蚜研究新进展[J].西北农林科技大学学报:自然科学版,2004,32(Z1):73-77.
[6] 钦俊德.昆虫与植物的关系-论昆虫与植物的相互作用及其演化[M].北京:科学出版社,1987:62-78.
[7] 夏云龙,杨奇华,萧红.冬小麦形态特征与抗麦长管蚜的关系研究[J].植物保护学报,1991,18(1):5-10.
[8] 段灿星,王晓鸣,朱振东,等.我国小麦抗麦长管蚜 Sitobion avenae 研究概况[J].植物遗传资源学报,2003,4(2):175-178.
[9] Rder M S,Korzun V,Wendehake K,et al.A microsatellite map of wheat[J].Genetics,1998,149(4):2007-2023.
[10] Plaschke J,Ganal M W,Roeder M S.Detection of genetic diversity in closely related bread wheat using microsatellite markers[J].Theoretical and Applied Genetics,1995,91(6-7):1001-1007.
[11] 倪中福,张义荣,梁荣奇,等.普通小麦D染色体组微卫星分子标记遗传差异研究[J].作物学报,2003,29(1):145-151.
[12] Peng J H,Bai Y,Haley S D,et al.Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russion wheat aphid[J].Genetica,2009,135(9):99-122.
[13] 史忠良,仇松英,张松令,等.小麦抗麦红吸浆虫品种的遗传多样性分析[J].农学学报,2011,1(1):10-16.
[14] 仇松英,张松令,史忠良,等.小麦抗蚜品种的遗传多样性SSR标记分析[J].农学学报,2011,1(2):5-10.
[15] 王春平,罗坤,赵惠燕,等.14份小麦种质资源抗麦长管蚜遗传多样性的SSR分析[J].核农学报,2011,25(4):639-644.
[16] 许兰杰,吕延华,段晓亮,等.孕穗期和灌浆期小麦品种(系)的抗蚜性评价[J].中国农业大学学报,2014,19(1):21-28.
[17] 蔡青年,张青文,高希武,等.小麦体内次生物质对麦蚜的抗性作用研究[J].中国农业科学,2003,36(8):910-915.
[18] Rder M,Plaschke J,Koenig S U,et al.Abundance,variability and chromosomal location of microsatellites in wheat [J].Molecular and General Genetics,1995,246(3):327-333.
[19] 刘炜,李自超,史延丽,等.利用SSR标记进行粳稻品种的遗传多样性研究[J].西南农业学报,2005,18(5):509-513.
[20] Anderson J A,Churchill G A,Autrique J E,et al.Optimizing parental selection for genetic linkage maps [J].Genome,1993,36(1):181-186.
[21] 赵瑞芳,侯本军,库丽霞,等.利用SSR标记分析12个玉米群体的遗传关系[J].玉米科学,2012,20(2):33-36,40.
[22] Zhang X Y,Li C W,Wang L F,et al.An estimation of the minimum number of SSR alleles needed to reveal genetic relationships in wheat varieties.I.Information from large-scale planted varieties and cornerstone breeding parents in Chinese wheat improvement and production [J].Theoretical and Applied Genetics,2002,106(1):112-117.
[23] 刘绍友,侯有明,李定旭,等.小麦品种对麦长管蚜抗性机制的研究[J].西北农业学报,1993,2(3):76-80.
[24] 陈巨莲,孙京瑞,丁红建,等.主要抗蚜小麦品种(系)的抗性类型及其生化抗性机制 [J].昆虫学报,1997,40(z1):190-195.
[25] Leszczynski B,Bakowski T,Rozbicka B,et al.Interaction between cereal phenolies and grain aphid(Sitobion avenae Fabr) [J].Bulletin OILB/SROP,1996,19(5):100-105.
[26] 刘勇,倪汉祥,孙京瑞,等.麦蚜对不同抗性小麦挥发物的嗅觉反应及其变异[J].中国农业科学,2001,34(4):391-395.
[27] 景蕊莲,昌小平.SSR标记在小麦种质资源研究中的应用[J].作物品种资源,1999(2):18-21.

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