华北农学报 ›› 2014, Vol. 29 ›› Issue (3): 159-163. doi: 10.7668/hbnxb.2014.03.029

所属专题: 畜牧 生物技术

• 论文 • 上一篇    下一篇

FASN基因与牦牛肌内脂肪酸组成的相关性研究

秦文1,2,3, 吴晓云1,2, 李天科1,2,3, 阎萍1,2   

  1. 1. 中国农业科学院 兰州畜牧与兽药研究所, 甘肃 兰州 730050;
    2. 甘肃省牦牛繁育工程重点实验室, 甘肃 兰州 730050;
    3. 甘肃农业大学 动物科学技术学院, 甘肃 兰州 730070
  • 收稿日期:2014-03-11 出版日期:2014-06-28
  • 通讯作者: 阎萍(1963-),女,山西运城人,研究员,博士生导师,主要从事牦牛育种研究。
  • 作者简介:秦文(1987-),女,甘肃庆阳人,在读硕士,主要从事动物遗传育种与繁殖研究。
  • 基金资助:
    国家肉牛牦牛产业技术体系牦牛选育项目(nycytx-38);甘肃牧区生产生态生活优化保障技术集成示范项目(2012BAD13B05)

Association of FASN Gene with Fatty Acid Composition in Yak

QIN Wen1,2,3, WU Xiao-yun1,2, LI Tian-ke1,2,3, YAN Ping1,2   

  1. 1. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China;
    2. Key Laboratory of Yak Breeding Engineering, Lanzhou 730050, China;
    3. Faculty of Animal Sci-tech Gansu Agricultural University, Lanzhou 730070, China
  • Received:2014-03-11 Published:2014-06-28

摘要: 为研究牦牛肌肉中脂肪酸组成特征及其形成机制,以牦牛和黄牛背最长肌为材料,利用气象色谱-质谱联用仪(GC-MS) 进行脂肪酸的定性定量分析,并通过Real-time PCR技术检测 FASN 基因在牦牛和黄牛背最长肌中mRNA的表达水平,同时分析了其与脂肪酸组成的相关性。结果发现,牦牛背最长肌中单不饱和脂肪酸(MUFA) 和多不饱和脂肪酸(PUFA) 含量均极显著高于黄牛(P< 0.01),而饱和脂肪酸(SFA) 显著低于黄牛(P< 0.05) 。牦牛背最长肌中 FASN 基因的mRNA表达水平极显著高于黄牛(P< 0.01) 。牦牛 FASN 基因mRNA表达量只与牦牛C10:0呈极显著正相关(P< 0.01),与黄牛脂肪酸组成无显著的相关性(P>0.05) 。

关键词: FASN基因, 牦牛, 黄牛, 表达量, 脂肪酸

Abstract: In order to study the characteristics and mechanism of fatty acid composition in yak, the fatty acids composition and content of Longissimus dorsi muscle in yak and cattle were determined by using gas chromatograph-mass spectrometer-computer(GC-MS), while the FASN gene mRNA expression level was investigated by Real-time PCR, and the correlation analysis was carried out.The results showed that the monounsaturated fatty acid(MUFA) and polyunsaturated fatty acids(PUFA) in yak was extremely significantly higher than in cattle(P< 0.01), while the saturated fatty acid(SFA) was significantly lower than in cattle(P< 0.05) .The FASN mRNA expression in yak was extremely significantly higher than in cattle(P< 0.01) .There is an extremely significant positive correlation(P< 0.01) between the FASN mRNA expression level and content of C10:0 in yak, no significant correlation(P>0.05) was found in cattle.

Key words: FASN gene, Yak, Cattle, Expression level, Fatty acid

中图分类号: 

引用本文

秦文, 吴晓云, 李天科, 阎萍. FASN基因与牦牛肌内脂肪酸组成的相关性研究[J]. 华北农学报, 2014, 29(3): 159-163. doi: 10.7668/hbnxb.2014.03.029.

QIN Wen, WU Xiao-yun, LI Tian-ke, YAN Ping. Association of FASN Gene with Fatty Acid Composition in Yak[J]. journal1, 2014, 29(3): 159-163. doi: 10.7668/hbnxb.2014.03.029.

使用本文

0
    /   /   推荐 /   导出引用

链接本文: http://www.hbnxb.net/CN/10.7668/hbnxb.2014.03.029

               http://www.hbnxb.net/CN/Y2014/V29/I3/159