7个<em>PcEXPA</em>基因在茌梨和黄金梨果实采后贮藏的表达调控研究

doi:10.7668/hbnxb.2014.03.028

华北农学报 ›› 2014, Vol. 29 ›› Issue (3): 153-158. doi: 10.7668/hbnxb.2014.03.028

所属专题： 梨；生物技术；

7个PcEXPA基因在茌梨和黄金梨果实采后贮藏的表达调控研究

杨绍兰, 张晓菲, 张新富, 王然

青岛农业大学园艺学院, 山东青岛 266109

收稿日期:2014-03-11 出版日期:2014-06-28
通讯作者: 王然（1960-），女，山东龙口人，教授，博士，主要从事果实采后生理与分子生物学研究。
作者简介:杨绍兰（1978-），女，山东招远人，副教授，博士，主要从事果实采后生理与分子生物学研究。
基金资助:
国家自然科学基金项目（31201608）；国家现代农业（梨）产业技术体系建设专项（nycytx-29-06）；山东省高等学校科技计划项目（J09LC13）；山东省优秀中青年科学家科研奖励基金项目（BS2010NY009）；青岛市科技计划基础研究项目（12-1-4-5-（8）-jch）；青岛农业大学高层次人才启动基金项目（630803）

The Expression Pattern of Seven PcEXPA Genes of Chili and Whangkeumbae Pear during Fruit Postharvest Storage

YANG Shao-lan, ZHANG Xiao-fei, ZHANG Xin-fu, WANG Ran

College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China

Received:2014-03-11 Published:2014-06-28

摘要/Abstract

摘要： 以茌梨和黄金梨果实为试材，研究了采后贮藏期间梨果实的硬度、乙烯释放速率、可溶性果胶含量以及7个 PcEXPA 基因的表达模式。结果表明，茌梨和黄金梨果实硬度在采后贮藏期间均呈下降趋势，但茌梨果实的硬度在贮藏后期明显高于黄金梨果实；黄金梨果实中的可溶性果胶含量明显高于茌梨果实；7个 PcEXPA 基因在2个品种梨果实中的表达模式显示，PcEXPA2 基因在茌梨果实和黄金梨果实中的表达与果实硬度变化和乙烯释放速率一致，且均受1-MCP下调，表明其可能参与了茌梨和黄金梨的成熟衰老进程。

关键词: 茌梨, 黄金梨, 软化, Expansin

Abstract: Chili and Whangkeumbae pear were used to examine the fruit firmness, ethylene production rate, soluble pectin content and the expression patterns of seven PcEXPA genes in this paper.During the postharvest storage, the fruit firmness of both Chili and Whangkeumbae pear were decreased, while Chili fruit maintained higher fruit firmness than Whangkeumbae fruit.The soluble pectin content of Whangkeumbae pear was higher than that of Chili pear.The expression of seven PcEXPA genes showed that the expression of PcEXPA2 was consistent with the changes of fruit firmness and the ethylene production rate, and were down-regulated by 1-MCP treatment in the two varieties of pear. PcEXPA2 was closely related to the fruit softening of Chili and Whangkeumbae pear.

Key words: Chili pear, Whangkeumbae pear, Softening, Expansin

中图分类号:

Q78
S661.01

杨绍兰, 张晓菲, 张新富, 王然. 7个PcEXPA基因在茌梨和黄金梨果实采后贮藏的表达调控研究[J]. 华北农学报, 2014, 29(3): 153-158. doi: 10.7668/hbnxb.2014.03.028.

YANG Shao-lan, ZHANG Xiao-fei, ZHANG Xin-fu, WANG Ran. The Expression Pattern of Seven PcEXPA Genes of Chili and Whangkeumbae Pear during Fruit Postharvest Storage[J]. journal1, 2014, 29(3): 153-158. doi: 10.7668/hbnxb.2014.03.028.

http://www.hbnxb.net/CN/Y2014/V29/I3/153

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7个PcEXPA基因在茌梨和黄金梨果实采后贮藏的表达调控研究

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