摘要： 为了筛选漆酶高产菌株,优化其液态发酵的培养基及培养条件。利用平板筛选获得菌株,通过测定漆酶活力优化培养基组成及培养条件。从自然界中筛选出一株产漆酶活力较高的白腐真菌,命名为J-1;菌株J-1适宜的发酵培养基为:麸皮4%,蛋白胨1.5%,CuSO41.0 mmol/L,对羟基苯甲酸10 mmol/L,氯化镁10 mmol/L;适宜的培养条件为:250 mL三角瓶中装40 mL培养基,培养基初始pH为5.0,接种量为6%,培养温度为30℃,转速为200 r/min,培养时间为9 d。

关键词: 白腐真菌, 漆酶, 筛选, 条件优化

Abstract: Isolation of high-yield strains for laccase production and optimization of the submerged fermentation medium and culture conditions.Screening strains were by medium flats and optimizing the composition of the medium was by measuring the laccase activity.a high yield fungus for laccase production was screened and named as J-1.The liquid fermentation medium and culture conditions for strain J-1 were optimized.The results were bran 4%,peptone 1.5%,CuSO4 1.0 mmol/L,hydroxy acid 10 mmol/L,magnesium chloride 10 mmol/L,250 mL flask with 40 mL medium,culture initial pH 5.0,inoculum 6%,culture temperature 30℃,speed 200 r/min and the culture time 9 d.

Key words: White-rot fungi, Laccase, Isolation, Optimization

中图分类号: 

• TQ925