小麦种质N9134抗白粉病基因的染色体臂定位

doi:10.7668/hbnxb.2011.06.008

摘要/Abstract

摘要： 明确来源于野生二粒小麦的抗白粉病基因PmAS846所在的染色体臂。采用端体和SSR标记对普通小麦种质N9134携带的PmAS846基因进行了遗传分析。中国春(Chinese Spring,CS)、CS5BL双端体系与N9134杂交F₁高抗白粉病,CS/N9134的F₂和CS5BL双端体系/N9134//CS BC1F₁抗病植株和感病植株比例分别符合期望比例3∶1和1∶1。CS 5BL双端体系/N9134//CS BC1F₁64个植株的根尖染色体核型和白粉病抗性鉴定发现,12个植株在5B染色体着丝点和抗性基因间发生了交换。PmAS846的SSR标记Xgwm67在CS第五部分同源群的缺体-四体系和双端体系的扩增,该标记在CSN5BT5D系中缺失,而在CSDt5BL系中存在。结果表明:PmAS846位于小麦5B染色体长臂上,该基因距5B染色体着丝点18.75个交换单位。

关键词: 野生二粒小麦, 抗白粉病基因, 染色体臂定位, SSR标记

Abstract: A powdery mildew resistance gene PmAS846derived from Triticum dicoccoidesaccession As846 and was located on 5B chromosome． The aim of this research was to locate this gene to chromosome arm． Genetic analysis of PmAS846transferred to common wheat line N9134 was done by ditelocentric line and SSR markers． F1 progeny between common wheat CS(Chinese Spring) disomics，CSDt5BL(CS 5BL ditelocentric line) and N9134 were highly resistant to powdery mildew． The ratio of the resistant and susceptible plants in CS /N9134 F2 progeny and CSDt5BL /N9134 / /CS BC1F1 progeny fitted the expected 3∶ 1 and 1∶ 1， respectively． The karyotype analysis of root tip cell and powdery mildew resistance for 64 CS5BL /N9134 / /CS BC1F1 plants was done，and the crossover types had frequencies of 12． The analysis results of SSR marker Xgwm67in CSN5AT5D，CSN5BT5D，CSN5DT5A，CSDt5AL， CSDt5BL and CSDt5DL indicated that the marker was absent in CSN5BT5D and present in CS Dt5BL． The results showed that PmAS846was located on 5BL and the distance of PmAS846and the 5B centromere was 18． 75 map units．

Key words: Triticum dicoccoides, Powdery mildew resistance gene, Chromosome arm location, SSR marker

中图分类号:

王长有, 王亚娟, 张宏, 刘新伦, 蔡东明, 吉万全. 小麦种质N9134抗白粉病基因的染色体臂定位[J]. 华北农学报, 2011, 26(6): 37-40. doi: 10.7668/hbnxb.2011.06.008.

WANG Chang-you, WANG Ya-juan, ZHANG Hong, LIU Xin-lun, CAI Dong-ming, JI Wan-quan. Chromosome Arm Location of Powdery Mildew Resistance Gene of Common Wheat Germplasm N9134[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 37-40. doi: 10.7668/hbnxb.2011.06.008.

http://www.hbnxb.net/CN/Y2011/V26/I6/37

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