华北农学报 ›› 2011, Vol. 26 ›› Issue (4): 198-201. doi: 10.7668/hbnxb.2011.04.035

所属专题: 油料作物

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花生黄曲霉抗性与类受体蛋白激酶的相关性分析

严海燕1, 宗成志1, 景聪慧1, 单世华2   

  1. 1. 中南民族大学生命科学学院, 湖北武汉 430074;
    2. 山东省花生研究所, 山东青岛 266100
  • 收稿日期:2011-03-15 出版日期:2011-08-28
  • 作者简介:严海燕(1961-),女,湖北人,教授,主要从事植物发育分子生物学研究工作。
  • 基金资助:
    与山东花生所的横向合作项目(HZY05013);国家自然科学基因项目(NSFC307711361)

Analysis of Relativity of Receptor-like Kinase to Aspergillus flavus Resistance in Peanut

YAN Hai-yan1, ZONG Cheng-zhi1, JING Cong-hui1, SHAN Shi-hua2   

  1. 1. College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China;
    2. Shandong Peanut Research Institute, Qingdao 266100, China
  • Received:2011-03-15 Published:2011-08-28

摘要: LRR类受体激酶RH4基因在花生黄曲霉敏感品种发育种子的种皮中上调表达。基于这类受体激酶多由于序列的变异导致抗性的变异,笔者将数据库中花生抗黄曲霉和敏感品种相似基因编码的蛋白进行序列比较分析,发现抗性品种与敏感品种的类似蛋白序列有很大差别。用荧光定量PCR方法对抗性品种KB153与敏感品种JH1012发育中不同时期的果实和部位进行差异表达分析,结果表明,果实发育的早期类受体蛋白激酶的大量表达可能是导致黄曲霉抗性的重要原因之一。

关键词: 黄曲霉, 花生, 类受体蛋白激酶, 抗性基因

Abstract: LRR type receptor like kinase(RLK) RHG is up-regulated in developing seed coat of sensitive strain relative to resistant strain to Aspergillus flavus.Based on the knowledgy that variation in sequences of LRR type RLK cause their function variety,we compared similar protein sequences encoded by ESTs from resistance and sensitive strains of Arachis hypogaea in public database,found that there were significant differences between protein sequences resistance and sensitive strains of Arachis hypogaea.We further conducted expression differential analysis on different tissues in different developing stages of both peanut Aspergillus flavus resistance and sensitive strains.The results indicate that up-regulation of expression of RLKis a possible reason of Aspergillus flavus resistance.

Key words: Aspergillus flavus, Arachis hypogaea, Receptor-like protein kinase, Resistance gene

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引用本文

严海燕, 宗成志, 景聪慧, 单世华. 花生黄曲霉抗性与类受体蛋白激酶的相关性分析[J]. 华北农学报, 2011, 26(4): 198-201. doi: 10.7668/hbnxb.2011.04.035.

YAN Hai-yan, ZONG Cheng-zhi, JING Cong-hui, SHAN Shi-hua. Analysis of Relativity of Receptor-like Kinase to Aspergillus flavus Resistance in Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 198-201. doi: 10.7668/hbnxb.2011.04.035.

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