摘要： 越来越多的研究表明,非生物胁迫影响植物的生长发育与栽培范围。为了通过转基因方法提高栽培番茄、马铃薯对热和干旱组合胁迫的抗性,利用RT-PCR结合RACE技术,从热处理的烟草叶片中分离出了细胞质小分子热激蛋白基因。分析表明,该基因cDNA全长876 bp,包含一个480 bp的开放阅读框,编码159个氨基酸,相对分子量约为17.8 kDa,被命名为Nt-HSP17.8。Northern杂交结果显示,该基因在烟草中的表达不仅受单一胁迫如热或干旱的诱导,而且在干旱与热组合胁迫下表达量大大增加,说明该基因在耐热与干旱交叉胁迫中起重要作用。
关键词:
烟草,
小分子量热激蛋白,
基因克隆,
基因表达
Abstract: Increasing evidences show that abiotic stresses can affect plant growth and development and the range of cultivation.In order to increase cultivating resistance of tomato and potato in the combination of heat and drought stresses through transgenic methods,cytoplasmic class I sHSPs cDNA with full length of 876 bp was cloned from heat-shocked leaves of Crofton weed(Nicotiana tabacum L.) by conducting RT-PCR and RACE-PCR.Sequence analysis showed that it included a 480 bp open reading frame(ORF) which encoded 159 amino acids with a deduced molecular weight of 17.8 kDa.It was called as Nt-HSP17.8.The results of Northern blot showed that expression of Nt-HSP17.8 gene in tobacco leaves was induced not only by a single stress such as heat or drought,but also by combination stress(heat shocK+ drought) conditions,indicating that the gene might play an important role in the combination of heat shock and drought stress.
Key words:
Nicotiana tabacum L．,
Small molecular weight heat shock protein,
Gene cloning,
Gene expression
中图分类号:
李春子, 成善汉. 烟草细胞质小分子热激蛋白基因Nt-Hsp17.8的克隆及胁迫诱导表达特性分析[J]. 华北农学报, 2011, 26(4): 202-206. doi: 10.7668/hbnxb.2011.04.036.
LI Chun-zi, CHENG Shan-han. Cloning of Small Molecular Shock Protein Gene Nt-Hsp17.8 from Nicotiana tabacum and Its Expression Analysis under Inuced by Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 202-206. doi: 10.7668/hbnxb.2011.04.036.