摘要： 蒙古牛是在蒙古高原的大陆性严寒气候条件下形成的一个古老的地方品种,它具有遗传性稳定、抗病耐粗饲、宜牧、抓膘能力强,适应性强等生物学特性。为了阐明BMP4基因对蒙古牛繁殖性能的影响,试验根据GenBank中报道的人、小鼠、绵羊和牛BMP4基因序列设计引物,从蒙古牛卵巢、输卵管、子宫、肾脏中提取RNA,以β-actin为内参照物,采用RT-PCR技术分析BMP4基因在蒙古牛卵巢、子宫、输卵管和肾脏组织中的表达情况。结果表明,BMP4基因在蒙古牛这4种组织中均有表达,只是在不同组织中的表达丰度不同。蒙古牛BMP4基因在卵巢的mRNA水平极显著高于在输卵管、子宫和肾脏(P<0.01);在子宫中的mRNA水平极显著高于输卵管和肾脏(P<0.01)。

关键词: 蒙古牛, BMP4, 组织表达, 半定量RT-PCR

Abstract: Mongolian cattle are ancient and local breed which were breed under cold weather condition of Mongolian Plateau.It possesses numerous outstanding characteristics such as,stable heredity,strong ability of disease resistance,coarse feed tolerance,grazing and fatten suitability,and great adaptability.In order to illustrate the influence of BMP4 gene to reproductive performance of Mongolian cattle,total RNA was isolated from the ovary,uterus,oviduct and kidney of Mongolian cattle,and the primers were designed according to the report by GenBank.Taking β-actin as inner Criteria,we detected the expression status of BMP4 gene in ovary,uterus,oviduct and kidney of Mongolian cattle by semi quantitative RT-PCR(SQ RT-PCR) technology in this research.The results show that BMP4 gene was expressed in ovaries,oviduct,uterus and kidney,but its expression abundances in different tissues was different.The mRNA level of BMP4 gene in the ovary of Mongolian cattle was extremely significantly higher than that in its oviduct,uterus and kidney(P0.01);and in the uterus was extremely significantly higher than that in the oviduct and kidney(P0.01).

Key words: Mongolian cattle, BMP4, Tissue expression, Semi-quantitative RT-PCR

中图分类号: 

• S823