华北农学报 ›› 2010, Vol. 25 ›› Issue (S1): 41-46. doi: 10.7668/hbnxb.20102.S1.011

所属专题: 甜瓜

• 论文 • 上一篇    下一篇

甜瓜育种亲本材料遗传多样性及群体结构的SSR标记分析

王美荣1,2, 许勇2, 詹永乐1, 郭绍贵2, 任毅2, 宫国义2, 张海英2   

  1. 1. 安徽农业大学生命科学院, 安徽 合肥 230036;
    2. 北京市农林科学院蔬菜研究中心, 北京 100097
  • 收稿日期:2010-04-22 出版日期:2010-12-30
  • 通讯作者: 张海英(1969-),女,博士,主要从事西甜瓜分子生物学研究
  • 作者简介:王美荣(1984-),女,硕士,主要从事西甜瓜资源研究
  • 基金资助:
    北京市科委项目(Z09090501040902;D08070500690803);国家现代西瓜甜瓜产业技术体系(nycytx-36-01-01-01);国家科技支撑项目(2006BAD01A7-6-02)

Analysis of Genentic Diversity and Population Structure in Melon (Cucumis melo L. ) Breeding Materials by SSR Markers

WANG Mei-rong1,2, XU Yong2, ZHAN Yong-le1, GUO Shao-gui2, REN Yi2, GONG Guo-yi2, ZHANG Hai-ying2   

  1. 1. Life Science College of Anhui Agricultural University, Hefei 230036, China;
    2. National Engineering Research Center for Vegetables,Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
  • Received:2010-04-22 Published:2010-12-30

摘要: 为明确供试甜瓜育种材料间的遗传关系,为育种工作提供有益信息,利用23对SSR引物对39份甜瓜育种材料(双亲、F1)进行遗传多样性和群体结构分析,共检测到71等位变异位点,平均每对引物产生等位变异数3.087个、Shannon-Weaver指数为0.893。遗传多样性和群体结构分析结果基本一致,供试育种材料分为厚皮甜瓜和薄皮甜瓜两大类群。群体结构分析还阐明了各育种材料的遗传组成,84.62%的育种材料血缘相对比较单一,15.38%的育种材料拥有混合来源。研究结果为亲本选配、优良育种材料利用提供了技术支持。

关键词: 甜瓜, 育种材料, SSR, 遗传多样性, 群体遗传结构

Abstract: To explore the genetic background of melon breeding materials,23 polymorphic Simple sequence repeat (SSR) markers were conducted to assess the genetic diversity and population structure among 39 melon breeding materials. 71 allelic variations were detected,the average allele number and Shannon-Weaver index of each primer were 3. 087 and 0. 893. Genetic diversity and population structure analyses revealed that results were basically same,39 melon accessions were clustered into two groups,Cucumis melo ssp. melo and C. melo ssp. conomon. The population structure analyses revealed the genetic composition of the 39 accessions,in which 84. 62% accessions shared a likely single ancestor,and the rest were from mixed ancestors. These results had provided valuable information for breeding and exploit of new melon accessions.

Key words: Melon, Breeding materials, SSR, Genetic diversity, Population genetic structure

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引用本文

王美荣, 许勇, 詹永乐, 郭绍贵, 任毅, 宫国义, 张海英. 甜瓜育种亲本材料遗传多样性及群体结构的SSR标记分析[J]. 华北农学报, 2010, 25(S1): 41-46. doi: 10.7668/hbnxb.20102.S1.011.

WANG Mei-rong, XU Yong, ZHAN Yong-le, GUO Shao-gui, REN Yi, GONG Guo-yi, ZHANG Hai-ying. Analysis of Genentic Diversity and Population Structure in Melon (Cucumis melo L. ) Breeding Materials by SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 41-46. doi: 10.7668/hbnxb.20102.S1.011.

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