华北农学报 ›› 2010, Vol. 25 ›› Issue (4): 218-220. doi: 10.7668/hbnxb.2010.04.046

所属专题: 甜瓜

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甜瓜根腐病病原分离与抗源鉴定

杨颖1,2, 耿丽华1, 王建设1, 宋晓华2   

  1. 1. 北京市农林科学院 蔬菜研究中心 北京 100097;
    2. 宁夏大学 农学院 宁夏 银川 750021
  • 收稿日期:2010-07-30 出版日期:2010-08-28
  • 通讯作者: 王建设(1966- ),男,宁夏青铜峡人,副研究员,博士,主要从事甜瓜遗传育种研究。
  • 作者简介:杨颖(1984- ),女,天津南开人,在读硕士,主要从事甜瓜遗传学研究.
  • 基金资助:
    北京市自然科学基金项目(5052012;6072012;6092008);国家自然科学基金项目(30871720)

Isolation of Root Rot Pathogen and Resistance Evaluation of Melon Materials

YANG Ying1,2, GENG Li-hua1, WANG Jian-she1, SONG Xiao-hua2   

  1. 1. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    2. Agronomy College, Ningxia University, Yinchuan 750021, China
  • Received:2010-07-30 Published:2010-08-28

摘要: 近年来根腐病在厚皮甜瓜设施与露地栽培中普遍发生,危害严重.试验对厚皮甜瓜根腐病病样进行病原菌分离和纯化,经培养、鉴定及致病性测定,证实分离的致病菌是甜瓜根腐病菌.以所获得的病原菌菌株作为菌种,制备病原接种体,采用幼苗离体接种法,对267份薄皮甜瓜种质资源进行根腐病抗性评价,结果筛选出16份高抗和20份抗病种质资源,说明薄皮甜瓜种质资源中蕴藏着对改良厚皮甜瓜根腐病抗性有潜在利用价值的基因资源.

关键词: 甜瓜, 根腐病, 病原分离, 抗源鉴定

Abstract: Resent years Melon root-rotten (,i>Cucumis melo) has been seriously happened in melon cultivation both in greenhouse and in field. We collected the typical diseased plants,isolated and identified the pathogen,the result of morphological investigations of the isolate from the diseased plants indicate that it is Fusarium solani(Mart.) Sacc. that causes Melon root-rotten. Use this pathogen to evaluate the resistance of 267 melon materials in lab,using seeding inoculation method. The result shows that 16 materials obtained from 267 C.melo var. chinensis materials were highly resistant to melon root-rotten; 20 materials were resistant. The result of seeding inoculation indicate there are available resistance resources in C.melo var. chinensis . This resistance resources could be used in melon root-rotten of melon resistance breeding.

Key words: C.melo, Root-rotten, Pathogen isolation, Resistance evaluation

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引用本文

杨颖, 耿丽华, 王建设, 宋晓华. 甜瓜根腐病病原分离与抗源鉴定[J]. 华北农学报, 2010, 25(4): 218-220. doi: 10.7668/hbnxb.2010.04.046.

YANG Ying, GENG Li-hua, WANG Jian-she, SONG Xiao-hua. Isolation of Root Rot Pathogen and Resistance Evaluation of Melon Materials[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 218-220. doi: 10.7668/hbnxb.2010.04.046.

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