摘要： 采用实时荧光定量PCR法,对小麦抗叶锈病近等基因系TcLr1接种亲和或非亲和性叶锈菌后,CaM及其亚型的mRNA表达差异进行相对定量分析.结果表明,小麦接菌后12 h内CaM 的4个亚型基因表达量与不接菌对照相比相差不大.小麦接种非亲和叶锈菌,48,72,96 h后,CaM SF-1表达量分别比亲和组合高 24.6%,26.6%,38.8%;接种24,48 h后,CaM SF-4表达量分别高出亲和组合26.8%和28.0%;在接种后24~96 h这一过程中,CaM SF-2表达量均低于亲和组合中, 而CaM SF-3表达量与亲和组合表达量相差不大.接种非亲和叶锈菌后,在第24 h 、第 48 h小麦CaM表达量分别高于亲和组合18.1% 和 47.9% ,而在第72 h和第96 h CaM表达量又低于亲和组合.上述结果暗示,CaM可能参与了小麦抗叶锈病反应,并且具有亚型特异性.小麦中CaM SF-1和 CaM SF-4可能与小麦抗叶锈病相关,CaM SF-2可能与小麦感叶锈病相关,而CaM SF-3可能不参与小麦抗叶锈病反应.

关键词: 小麦, 叶锈菌, 钙调素, 亚型, 实时荧光定量PCR

Abstract: CaM is involved in plant resistance response. To study the differential expression of CaM and four isforms gene in wheat TcLr15 after inoculation with virulent race and avirulent race，r elatively quantitative analysis was performed by real-time PCR. The level of CaM and four CaM isforms gene expression in wheat TcLr15 was tested by real-time Fluorescent Quantitative PCR (real-time PCR) after inoculation with virulent race and avirulent race respectively. No significant difference in the gene expression of CaM and its four isoforms was observed between the detached wheat leaves inoculated with virulent and without until 12 h after inoculation. The gene expression of CaM SF-1 in wheat at 48 h，72h and 96 h after inoculation with avirulent race 05-5-127 ③ were increased by 24.6%，26.6% and 38. 8% compared with that of inoculation virulent race 05-22-64 ①. The gene expression of,CaM SF-4 at 24 h， 48 h after inoculation with avirulent race 05-5-127 ③ were increased by 26. 8% and 28. 0% if compared to that of inoculation with viruent race 05-22-64 ①. The gene expression of CaM SF-2 in the incompatible interaction was lower than that of compatible interaction control from 24 h to 96 h after inoculation. No obvious difference in gene expression level of CaM SF-3 between compatible and incompatible interaction was found. The gene expression of CaM in wheat after inoculation with avirulent race 05-5-127 ③ at the 24h and 48h were increased by 18.1% and 47.9% if compared to that of inoculation with virulent race 05-22-64 ①，however，the gene expressive quantity of CaM in wheat at 72 h and 96 h after inoculation with avirulent race 05-5-127 ③ was lower than that of inoculation with virulent race 05-22- 64 ①. Taken together，these data implied that CaM might be involved in the regulation on wheat resistance against its leaf rust disease. More over，there exist a CaM isoform specificity for the involving in wheat defense against the infection of Puccinia triticina. The CaM SF-1 and CaM SF-4 genes may be related to wheat against the infection of puccinia triticina. The CaM SF-2 gene may be related to wheat susceptible to the infection of Puccinia triticina. The CaM SF-3 gene may be not related to wheat against the infection of puccinia triticina.

Key words: Wheat, Puccinia triticina, CaM, Isforms, Real-time PCR

中图分类号: 

• S432.2+3