苦参黄酮对黄瓜枯萎病及抗性生理的影响

doi:10.7668/hbnxb.2009.06.016

摘要/Abstract

摘要： 采用溶剂萃取法提取苦参活性成分黄酮物质,研究不同浓度的苦参黄酮对黄瓜枯萎菌菌丝生长、抗病效果及抗性生理的影响.结果表明,苦参黄酮对黄瓜枯萎菌菌丝表现为各浓度(0.2,0.,1,2,4 g/L)下均具有抑制作用,作用强度随着浓度增加而增强,4 g/L苦参黄酮处理,菌丝抑制率达到93.08%.在治疗处理中,连续施用60 mg/L苦参黄酮效果最好,叶绿素含量、根系活力、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和苯丙氨酸解氨酶(PAL)活性,分别比对照提高17.79%,71.%,48.20%,123.80%,11.48%,丙二醛(MDA)含量和相对电导率较对照降低了60.43%和40.7%.在预防处理中,各浓度处理均提高了黄瓜植株的抗病性,其中以80 g/L处理抗病性最强.

关键词: 苦参, 黄酮, 枯萎菌, 抗性生理

Abstract: Flavonoids of Sophora flavescens were extracted with solvent extraction on mycelial growth of Fusarium oxysporum f.sp.cucumerinum,disease resisting effect and resistance physiology with different concentrations were studied.The results showed that all the concentrations(0.25,0.5,1,2,4 g/L) of flavonoids of Sophora flavescens restrained the mycelial growth of Fusarium oxysporum f.sp.cucumerinum,and the stimulative rates were enhanced with the increased concentration.As the concentration of flavonoids was 4 g/L,the inhibition rate was 93.08%.In the therapeutic trail,plants have the biggest promtion by 60 g/L flavonoidswhich results indicated that chlorophyll content,root activity,superoxide dismutase(SOD) activity,peroxidase(POD) activity and phenylalanine ammonia-lyase(PAL) activity were increased 17.79%,71.55%,48.20%,123.80%,151.48 %comparing with control.Malondialdehyde(MDA) content and relative conductivity declined 62.33%and 71.14%comparing with control.The results of the prevention trail indicated that flavonoids of Sophora flavescens enhanced the disease resistance of cucumber at all the concentrations,and the best effect present in 80 g/L.

Key words: Sophora flavescens, Flavonoids, Fusarium oxysporum f.sp., Resistance physiology

中图分类号:

周宝利, 姚婷, 张健, 叶雪凌. 苦参黄酮对黄瓜枯萎病及抗性生理的影响[J]. 华北农学报, 2009, 24(6): 78-82. doi: 10.7668/hbnxb.2009.06.016.

ZHOU Bao-li, YAO Ting, ZHANG Jian, YE Xue-ling. Effects of Flavonoids of Sophora flavescens to Fusarium Wilt and Resistance Physiology of Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 78-82. doi: 10.7668/hbnxb.2009.06.016.

http://www.hbnxb.net/CN/Y2009/V24/I6/78

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