滁菊<em>f3'h</em>基因启动子的克隆及分析

doi:10.7668/hbnxb.2016.S1.011

摘要/Abstract

摘要： 为探明滁菊类黄酮3'-羟化酶（F3'H）基因在类黄酮物质合成过程中的作用，基于本实验室已经获得滁菊f3'h编码基因的基础上，采用Genome Walking技术，克隆了1 348 bp的启动子，序列分析表明，该启动子片段除含有真核生物启动子核心元件TATA-Box等外，还含有参与脱落酸响应（ABRE）元件以及G-Box等参与光响应单元、植物激素响应单元件植物防御和逆境胁迫应答元件、以及环境因素等多个顺式调控元件。该基因启动子的克隆及其初步分析将为深入研究f3'h基因在滁菊类黄酮合成中的分子机制提供参考、为开发滁菊分子育种提供有效基因原件，以及为今后利用生物技术手段提高滁菊黄酮含量奠定基础。

关键词: 滁菊, 启动子, Genome Walking, 类黄酮, f3'h基因

Abstract: Flavonoid 3'-hydroxylase (F3'H) plays important roles in flavonoids synthesis pathway.In previous research, f3'h gene from (Dendranthema morifolium) Chuju was cloned in our laboratory.For further elucidating the function,by using genome walking technology,its promoter of 1 348 bp in length was cloned.Sequence analysis showed that besides eukaryotic core promoter element TATA-Box, the promoter fragment also contains multiple cis-regulatory elements, such as the element involved in abscisic acid response (ABRE), G-Box participating in the optical response unit, plant hormone response unit, plant defense and stress response element, and environmental factors, etc. The cloning of gene promoter paves the way for the further research on the molecular mechanism of f3'h gene in the synthesis of flavonoids in Chrysanthemum,provides effective components for engineering Chrysanthemum to improve the content of flavonoids.

Key words: Chuju, Promoter, Genome Walking, Flavonoids, f3'h gene

中图分类号:

赵维萍, 陆李昌, 刘洋, 徐媛媛, 翟璐, 蔡华. 滁菊f3'h基因启动子的克隆及分析[J]. 华北农学报, 2016, 31(S1): 63-67. doi: 10.7668/hbnxb.2016.S1.011.

ZHAO Weiping, LU Lichang, LIU Yang, XU Yuanyuan, ZHAI Lu, CAI Hua. Cloning and Sequence Analysis of the Promoter of f3'h Gene in Chuju[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 63-67. doi: 10.7668/hbnxb.2016.S1.011.

http://www.hbnxb.net/CN/Y2016/V31/IS1/63

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滁菊f3'h基因启动子的克隆及分析

Cloning and Sequence Analysis of the Promoter of f3'h Gene in Chuju

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滁菊f3'h基因启动子的克隆及分析

Cloning and Sequence Analysis of the Promoter of f3'h Gene in Chuju

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