摘要： 利用PCR技术扩增出牛结核杆菌mpb64基因片段，克隆到真核载体pcDNA3．1(+)上，构建重组质粒pCM64，将该重组质粒转染SP2/0细胞，间接免疫荧光试验和Western blotting检测目的基因的表达情况。结果表明，在转染了重组质粒pCM64的SP2/0细胞中出现绿色荧光，转染的SP2/0细胞泳道23 kDa处出现特异条带，说明mpb64基因在SP2/0细胞中成功进行了瞬时表达。

关键词: 牛结核杆菌, mpb64基因, 表达

Abstract: The mpb64 gene fragment amplified by PCR from Mycobacterium bovis was cloned into the eukaryotic expression vector pcDNA3.1(+),and the recombinant plasmid pCM64 was obtained.Then the recombinant plasmid was transfected into SP2/0 cells in vitro.The expression of target gene was detected by indirect immunofluorescence and Western blotting.The result showed that the green fluorescence appeared in the SP2/0 cells transfected by pCM64 and the specific band about 23 kDa appeared in the electophoretogram of SP2/0 cells,indicaing that the target gene mpb64 had been successfully expressed.

Key words: Mycobacterium bovis, mpb64 gene, Expression

中图分类号: 

• S832