摘要： 为从分子生物学角度进一步揭示玉米对灰斑病抗性的生理机制提供理论依据,利用紫外可见分光光度计,采用比色(酶活测定)法,研究了玉米灰斑病菌侵入对不同抗性玉米品种保护酶活性的影响.结果表明,玉米灰斑病菌侵入后不同抗性玉米品种体内的保护酶活性均发生一定的变化:POD活性增加、CAT活性降低,酶活增加(或降低)的幅度和提高(或降低)率与寄主的抗感性关系不密切;EST活性提高,抗病品种酶活增加的幅度和提高率比感病品种高;SOD活性增加,且感病品种SOD酶活增加的幅度比抗病品种高;PPO活性增加,感病品种酶活提高率较高,而中抗和高抗病品种酶活提高率较低.说明玉米在遭到病菌侵入时,体内会产生相应的反应来减轻危害.

关键词: 玉米品种, 玉米灰斑病菌, 保护酶

Abstract: It indicated that when corn was hurt, the body would develop appropriate response to mitigate damage.Us - ing the method of chromometry by ultraviole- t visible spectrophotometer, the effect on the activity of protective enzymes in different resistant maize varieties infected by the gray leaf spot pathogens were analyzed.The results showed that the activ - ity of some protective enzymes changed, including:the activity of POD was increased, the activity of CAT was decreased, both changes of the two enzyme activity were not related with the host resistance; the activity of EST was increased, fur - thermore both the increase rate and extent of the enzyme activity in disease -resistant varietieswere higher than that in sus - ceptible varieties; the activity of SOD was increased, the increase rate and extent of the activity in disease -susceptible va - rieties were higher than that in resistant varieties; the activity of PPOwas increased, the elevation rate of the PPO activity in susceptible varieties were higher, rather than in middling and high disease -resistant varieties.

Key words: Corn varieties, Corn gray leaf spot pathogens, Protective enzymes

中图分类号: 

• S453.13