摘要： 通过CO2加富、高温处理,对温室嫁接黄瓜幼苗的细胞膜透性及保护酶活性等内在生理指标进行测定分析,旨在为黄瓜抗热高产的理论提供理论依据。结果表明,经高温处理,嫁接黄瓜幼苗的质膜透性、脯氨酸(Pro) 、丙二醛(MDA) 含量明显增加,且高于对照,差异极显著;超氧化物歧化酶(SOD) 、过氧化物酶(POD) 、过氧化氢酶(CAT) 活性均呈先增后减变化趋势,变化极明显。CO2加富、高温处理,嫁接黄瓜幼苗的质膜透性、Pro、MDA含量均呈先增后减的趋势,在处理第6天低于对照;SOD、POD、CAT活性均呈上升趋势,且比高温处理要强。CO2加富、常温处理,温室嫁接黄瓜幼苗的质膜透性、Pro、MDA含量均低于对照,SOD、POD、CAT活性均高于对照,且低于高温处理。说明增加CO2浓度,有效地减弱了高温对嫁接黄瓜幼苗叶片引起的伤害,提高了嫁接黄瓜植株防御高温的能力,对温室嫁接黄瓜耐热性具有促进作用。
关键词:
CO2,
高温,
嫁接黄瓜,
质膜透性,
保护酶
Abstract: By CO2 enrichment, high temperature, greenhouse cucumber seedlings grafted membrane permeability, a nd protective enzyme activities were measured indicators such as internal physiological analysis to the theoretical yield of cucumber heat and provide a theoretical basis. This study suggested that the permeability of membrane, proline content, malondladehyde content of grafting cucumber seedlings in the greenhouse increased obviously with high temperature, and were higher than the contrast with statistical differences; SOD activity, POD activity, CAT activity increased at first but decreased later with significant modification; It showed a tendency with increase at first and decrease later of the permeability of membrane, proline content, malondladehyde content of grafting cucumber seedlings in the greenhouse within high temperature and elevated CO2, a nd was lower than the contrast at the sixth day of conduct; SOD activity, POD activity, CAT activity showed a tendency with increase and was greater compared with the zones of high temperature; the permeability of membrane, proline content 、malondladehyde content of grafting cucumber seedlings were lower than the contrast with elevated CO2, SOD activity, POD activity, CAT activity were higher than the contrast but were lower than the zones of high temperature. From the data it is concluded that CO2 enrichment not only receded the injury caused by the high temperature, but also could enhance the ability of the plants to resist high temperature, heat resistance of cucumber in greenhouse grafted have a catalytic effect.
Key words:
CO2,
High-temperature,
Grafting cucumber,
Permeability of membrane,
Protective enzymes
中图分类号:
王玉静, 崔世茂, 方浩, 刘杰才, 杨志刚, 付崇毅, 陈源闽. CO2加富、高温处理对温室嫁接黄瓜幼苗质膜透性和保护酶活性的影响[J]. 华北农学报, 2012, 27(1): 159-163. doi: 10.3969/j.issn.1000-7091.2012.01.030.
WANG Yu-jing, CUI Shi-mao, FANG Hao, LIU Jie-cai, YANG Zhi-gang, FU Chong-yi, CHEN Yuan-min. Influence of Elevated CO2 and High Temperature on the Permeability of Membrane and Protective Enzymes of Grafting Cucumber Seedlings in the Greenhouse[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 159-163. doi: 10.3969/j.issn.1000-7091.2012.01.030.