小麦热胁迫相关蛋白<em>Hsa32</em>基因的克隆

doi:10.7668/hbnxb.2008.06.001

华北农学报 ›› 2008, Vol. 23 ›› Issue (6): 1-5. doi: 10.7668/hbnxb.2008.06.001

所属专题： 小麦；生物技术；

• 论文 • 下一篇

小麦热胁迫相关蛋白Hsa32基因的克隆

李国良, 张红梅, 周人纲

河北省农林科学院, 遗传生理研究所, 河北省植物转基因中心, 河北, 石家庄, 050051

收稿日期:2008-10-28 出版日期:2008-12-28
作者简介:李国良(1979－)，男，河北丰润人，助理研究员，主要从事植物抗逆生理研究。
基金资助:
河北省自然科学基金(C2004000726)；河北省农林科学院青年基金(A06060102)

Cloning of a Heat-stress-associated Protein Hsa32 Gene from Wheat

LI Guo-liang, ZHANG Hong-mei, ZHOU Ren-gang

Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

Received:2008-10-28 Published:2008-12-28

摘要/Abstract

摘要： 热胁迫相关蛋白Hsa32主要存在于陆生植物中，Hsa32参与植物获得性耐热性的维持。本研究以37℃热胁迫1 h的小麦幼苗叶子为材料，提取总RNA，结合RT－PCR和RACE的方法克隆获得小麦Hsa32基因，该基因完整的开放阅读框全长88 bp，编码294个氨基酸。编码的氨基酸与水稻中Hsa32(OsHsa32)同一性最高，达83%，与拟南芥中Hsa32(AtHsa32)有66%的同一性，与番茄中Hsa32(LeHsa32)有63%的同一性，由此将其命名为TaHsa32。Northern杂交结果表明，TaHsa32是一个诱导型热激蛋白。

关键词: 小麦, 热激蛋白, Hsa32, 克隆

Abstract: Hsa32 (heat-stress-associated 322kDa protein) is mainly found in land plants and takes part in acquired thermotolerance in plants. A novel Hsa32 protein gene was cloned from wheat (Triticum aestivum L.) after heat stress 1 h at 37℃using RT-PCR and RACE(rapid amplification of cDNA ends) technology,and named as TaHsa32. The complete open reading frame (ORF)of the TaHsa32cDNA was 885 bp and encoding a protein of 294 amino acid residues. The comparison of the deduced amino acid sequences of the TaHsa32 with OsHsa32 (from rice),AtHsa32 (from Arabidopsis) and LeHsa32 (from tomato) showed that this was a highly conserved protein and the identity was 83 %,66%,63%,respectively. Using Northern blot analysis it was found that the expression of TaHsa32 gene was a induced by heat stress.

Key words: Wheat, Heat shock protein, Hsa32, Cloning

中图分类号:

Q945

李国良, 张红梅, 周人纲. 小麦热胁迫相关蛋白Hsa32基因的克隆[J]. 华北农学报, 2008, 23(6): 1-5. doi: 10.7668/hbnxb.2008.06.001.

LI Guo-liang, ZHANG Hong-mei, ZHOU Ren-gang. Cloning of a Heat-stress-associated Protein Hsa32 Gene from Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 1-5. doi: 10.7668/hbnxb.2008.06.001.

http://www.hbnxb.net/CN/Y2008/V23/I6/1

参考文献

[1] Lindquist S.The heat-shock response[J].Ann Rev Biochem,1986,55:1151-1191.
[2] Georgopoulos C,Welch W J.Role of the major heat shock proteins as molecular chaperones[J].Annu Rev Cell Biol,1993,9:601-634.
[3] Lee G J,Vierling E.A small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein[J].Plant Physiol,2000,122:189-198.
[4] Hartl F U,Hayer-Hartl M.Molecular chaperones in the cytosol:from nascent chain to folded protein[J].Science,2002,295:1852-1858.
[5] Waters E R,Lee G J,Vierling E.Evolution,structure and function of the small heat shock proteins in plants[J].J Biol Chem,1996,47:325-338.
[6] Nover L,Bharti K,Doring P,et al.Arabidopsis and the heat stress transcription factor world:how many heat stress transcription factors do we need[J].Cell Stress Chaperones,2001,6:177-189.
[7] Lohmann C,Eggers-Schumacher G,Wunderlich M,et al.Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis[J].Mol Gen Genomics,2004,271:11-21.
[8] Busch W,Wunderlich M,Schoffl F.Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana[J].Plant J,2008,41:1-14.
[9] Schramm F,Larkindale J,Kiehlmann E,et al.A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis[J].Plant J,2008,53:264-274.
[10] Yoshida T,Sakuma Y,Todaka D,et al.Functional analysis of an Arabidopsis heat-shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress-regulatory system[J].Biochemical and Biophysical Res Communications,2008,368:515-521.
[11] Charng Y Y,Liu H C,Liu N Y,et al.A heat-inducible transcription factor,HsfA2,is required for extension of acquired thermotolerance in Arabidopsis[J].Plant Physiol,2007,143:251-262.
[12] Queitsch C,Hong S W,Vierling E,et al.Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis[J].Plant Cell,2000,12:479-492.
[13] Su P H,Li H M.Arabidopsis stromal 70-kDa heat shock proteins are essential for plant development and important for thermotolerance of germinating seeds[J].Plant Physiol,2008,146:1231-1241.
[14] Dafny-Yelin M,Tzfira T,Vainstein A,et al.Non-redundant functions of sHSP-CIs in acquired thermotolerance and their role in early seed development in Arabidopsis[J].Plant Mol Biol,2008,67:363-373.
[15] Li G L,Chang H,Li B,et al.The roles of the atDjA2 and atDjA3 molecular chaperone proteins in improving thermotolerance of Arabidopsis thaliana seedlings[J].Plant Science,2007,173:408-416.
[16] Liu N Y,Ko S S,Yeh K C,et al.Isolation and characterization of tomato Hsa32 encoding a novel heat-shock protein[J].Plant Sci,2006,170:976-985.
[17] Charng Y Y,Liu H C,Liu N Y,et al.Arabidopsis Hsa32,a novel heat shock protein,is essential for acquired thermotolerance during long recovery after acclimation[J].Plant Physiol,2006,140:1297-1350.
[18] Sambrook J,Russell D W.Molecular cloning:a laboratory mannual[M].3rd ed.Cold Spring Harbor Laborotry Press,2001:150-153.

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小麦热胁迫相关蛋白Hsa32基因的克隆

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