摘要： 为保护野生沙葱种质资源,探明其种子的耐盐能力及人工驯化栽培中适宜的土壤条件,以不同浓度NaCl溶液处理沙葱种子,研究了盐胁迫对沙葱种子生活力及抗氧化酶活性的影响。沙葱种子在20~500 mmol/L浓度的NaCl胁迫处理下,发芽率、发芽势和发芽指数都随着溶液浓度的增加而降低,除20 mmol/L处理外,其他处理均达到显著差异,其中各处理的盐害率较对照分别高13.7%~99.0%;在20和50 mmol/L的盐胁迫处理下,萌发的沙葱种子SOD、POD活性均显著高于对照,大于100 mmol/L的处理活性均低于对照;CAT活性则随NaCl浓度增加而逐渐升高。在20和50 mmol/L浓度的NaCl胁迫处理下,沙葱种子MDA含量略低于对照,而当NaCl溶液浓度大于100 mmol/L时MDA含量随浓度的增加呈增加趋势,且各个处理的MDA含量均显著高于对照。沙葱种子适宜在NaCl溶液浓度小于100mmol/L条件下萌发。

关键词: 沙葱, 盐胁迫, 种子生活力, 抗氧化酶

Abstract: To protect the germplasm resource of wild Allium mongolicum, prove up the ability for bearing the salt and the feasible condition of domesticating planting. This paper studied the effect of NaCl stress on vigor and activities of an - tioxidant enzymes in Allium mongolicum seed, which the treatments are different salt concentrations. The results showed that:With the increasing of salt concentrations from 20 to 500 mmol/ L, the germination rate, all of germination energy and germination index was decreased significantly except of 20mmol/ L salt treatment, the salt injury ratio of every treatments was increased 13.7%- 99.0% than the control.The SOD and POD activities were significantly higher than the control under 20 and 50mmol/ L salt treatments, but lower when the salt concentrations is above of 100 mmol/ L.The CAT activ- i ty was increased gradually with the increasing of salt concentration. Under NaCl treatments with 20 and 50 mmol/ L, the content of MDA of Allium mongolicum R.Seed is a little lower than that of the control, but increasedwith the increased of the salt concentrations above of 100mmol/L.And the MDA contents of every treatments were all higher than the control. According to above,The Allium mongolicum R. seeds adapt to bourgeon under the salt treatments lower than 100mmol/L.

Key words: Allium mongolicum Regel., Salt stress, Seeds vigor, Antioxidants enzyme

中图分类号: 

• S649