摘要： 草莓灰霉病菌(Botrytis Ciennea Persoon)在 21～25℃下 pH 3～4的霉菌生长培养基中培养产生毒素,致使草莓愈伤组织细胞的活性丧失。产生毒素的高峰期在静置培养20 d,培养滤液用氯仿萃取,所得粗提物稀释90倍后仍有毒性存在,30倍稀释的毒素溶液处理愈伤组织,用荧光法观察,1.0h细胞致死率达86％,2.5h细胞致死率高达99.9％。

关键词: 草莓灰霉病菌, 毒素, 荧光

Abstract: At temperature of 21-25?,in substrate of pH 3-4,grey mould germ of straw berry produced mycotoxin, and the toxin can cause wound callus of straw berry to inactivate. Summit of mycotoxin was the 20 days. Cultivated liquid was leached, and then extracted with chloroform. The extractive which was diluted to 90 times still remained its toxicity. Wound callus was treated with mycotoxin solution which was diluted to 30 times,and then observed with fluorometry,mor-tality to cell was 86% in an hour and it was 99.9% in 2.5 hours.

Key words: Grey mould germ of straw berry, Toxin, Fluorometry

中图分类号: 

• S436.639