摘要： 普通小麦比较容易与硬粒小麦-簇毛麦双二倍体TH₁和 TH₁W杂交,所选的9个普通小麦品种或品系与TH₁和 TH₁W的 19个杂交组合,平均结实率为46.7％,共计获得19个杂交组合2316粒杂种种子。正反交结果表明,以普通小麦为母本的杂交结实率高于反交结实率,但是在杂种幼胚愈伤组织诱导率受正反交的影响小于杂交结实率。在继代培养过程中,杂种幼胚愈伤组织生长迅速,甚至直接诱导出绿苗,共获得不同杂交组合2005株再生植株,移栽成活率达到95％以上。观察还发现有少量再生植株发生形态变异,但是组织培养对提高普通小麦×硬粒小麦-簇毛麦双二倍体杂种育性没有明显的作用。

关键词: 普通小麦, 硬粒小麦-簇毛麦双二倍体, 杂文, 幼胚培养

Abstract: It was easy to cross wheat with T. durum-D. villosum amphidiploid cv. TH1 and TH1W. The average percentage of seed set among 19 crosses between nine wheat varieties or bred lines selected with TH1 and TH1W was 46.7% . A total of 2316 hybrid seeds were brought about. The results of reciprocal crosses revealed that higher seed set was completed with wheat as mater-nal parent. However, the margin between reciprocal crosses were smaller in callus induction per-centage than in seed set percentage. Calli originated from immature embryos of hybrids propagated vigorously,and even differentiated plantlets directly during subculture. Two thousand and five re-generants were attained in total. The survival rate of field transplantation was higher than 95% . Observation also indicated that morphological variations were seen in few regenerats. Tissue cul-ture Had no obvious effect on improvement of fertility of T. aestivum ?T. durum-D. ?illosum amphidiploid.

Key words: Triticum aestivum T., durum-Dasypyrum villosum amphidiploid Hybridization Immature embryo culture

中图分类号: 

• S512.1