华北农学报

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洋葱胞质雄性不育系及其保持系花蕾内源激素含量和脯氨酸含量的动态变化特征

曾爱松1, 梁毅2, 严继勇1, 李健绮1, 宋立晓1, 高兵1   

  1. 1. 江苏省农业科学院蔬菜研究所, 江苏 南京 210014;
    2. 北京农林科学院蔬菜研究中心, 北京 100097
  • 收稿日期:2012-08-01 出版日期:2012-12-31
  • 通讯作者: 严继勇 (1963 - ), 男, 江苏姜堰人, 研究员, 博士, 主要从事甘蓝 、 洋葱遗传育种研究;梁 毅(1969 - ), 男, 河南罗山人, 副研究员, 硕士, 主要从事洋葱与胡萝卜遗传育种研究。
  • 作者简介:曾爱松(1977 - ), 女, 山东菏泽人, 助理研究员, 硕士, 主要从事洋葱 、 甘蓝遗传与生物技术育种研究。
  • 基金资助:
    农业部公益性行业(农业)科研专项经费资助项目(200903018)

Dynamic Changes of Endogenous Hormones and Free Proline in Cytoplasmic Male Sterile Lines and Maintainer Lines of Onion ( Allium cepa L. )

ZENG Ai-song1, LIANG Yi2, YAN Ji-yong1, LI Jian-qi1, SONG Li-xiao1, GAO Bing1   

  1. 1. Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;
    2. Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China
  • Received:2012-08-01 Published:2012-12-31

摘要: 以洋葱胞质雄性不育系63A及其同核异质保持系63B为试材,研究了花蕾发育过程中IAA、GA3、ZR、ABA含量以及脯氨酸含量的动态变化规律,探讨洋葱不育性与内源激素、脯氨酸含量的关系。结果显示,洋葱不育系花蕾的IAA、GA3、ZR和脯氨酸含量在败育过程中显著低于保持系,而ABA含量则较保持系有不同程度的盈积。且在花药的发育过程中,二者的差异主要发生在单核期,即不育系小孢子败育的主要时期。据此认为,IAA、GA3、ZR、ABA和脯氨酸含量的异常与洋葱雄性不育的发生有关。

关键词: 洋葱, 雄性不育, 内源激素, 脯氨酸

Abstract: Onion ( Allium cepa L. ) buds of cytoplasmic male-sterile( CMS) line and its maintainer line at different developmental stages were employed as experimental materials to reveal relationships between CMS and changes of endogenous hormone and prolin contents by measuring IAA, GA3 , ZR, ABA and free prolin contents. The results showed that in course of abortion, sterile buds were lower significantly in IAA, GA3 , ZR and free prolin contents, while higher in ABA contents. In anthers development stages, the difference happenned mainly in the mainpollen abortion stage( monokaryophase) . It is suggested that the contents of IAA, GA3 , ZR, ABA , and free prolinmay be related with the occurrence of male-sterile onion.

Key words: Onion, Male sterile, Endogenous hormone, Prolin

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引用本文

ZENG Ai-song, LIANG Yi, YAN Ji-yong, LI Jian-qi, SONG Li-xiao, GAO Bing. Dynamic Changes of Endogenous Hormones and Free Proline in Cytoplasmic Male Sterile Lines and Maintainer Lines of Onion ( Allium cepa L. )[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.016.

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