大白菜育性相关蛋白差异表达

doi:10.7668/hbnxb.2015.02.023

摘要/Abstract

摘要： 为揭示复等位基因遗传的大白菜雄性不育分子机制,通过基于质谱的蛋白质组学同位素相对标记与绝对定量技术技术(iTRAQ),开展了大白菜核基因雄性不育蛋白质组学研究,以找到不育与可育材料中差异表达蛋白,从蛋白水平来进一步揭示大白菜雄性不育的分子遗传机制。通过研究,共发现了358个差异蛋白,其中可育中上调表达的蛋白有226个,下调表达的蛋白有132个,GO分析结果表明,鉴定的蛋白质组数据具有较好的生物学功能覆盖范围。通过双向电泳验证,差异蛋白点差异特性与iTRAQ结果类似,表明iTRAQ用于差异蛋白分析结果可靠。

关键词: 大白菜, 核基因雄性不育, iTRAQ, 差异表达蛋白

Abstract: To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa L.ssp pekinensis), the iTRAQ quantitation technique based on mass spectrometry were used to study the differencial expression between proteins fertile- and sterile-buds.This research would provide the basis for analyzing the molecular mechanism of multiple-allele-inherited male sterility in Chinese cabbage.In this research, a total of 358 differencial expressed proteins (226 up-expressed and 132 down-expressed proteins in fertile buds) were found and Gene Ontology (GO) analysis showed that the proteins identified data has better coverage of biological functions.It is verified by two-dimensional electrophoresis (2-DE).This results is consistent with iTRAQ technique, which indicated that using iTRAQ to analyze protein profile is reliable.

Key words: Chinese cabbage, Male sterility, iTRAQ, Differential proteomics

中图分类号:

Q78
S634.03

毕青, 周雪, 冀瑞琴, 冯辉. 大白菜育性相关蛋白差异表达[J]. 华北农学报, 2015, 30(2): 128-131. doi: 10.7668/hbnxb.2015.02.023.

BI Qing, ZHOU Xue, JI Rui-qin, FENG Hui. Differencial Expression of Fertility-related Proteins in Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 128-131. doi: 10.7668/hbnxb.2015.02.023.

http://www.hbnxb.net/CN/Y2015/V30/I2/128

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