玉米烟嘧磺隆敏感基因的遗传分析及基因定位

doi:10.3969/j.issn.1000-7091.2012.04.028

摘要/Abstract

摘要： 磺酰脲类除草剂烟嘧磺隆由于低用量、低毒、广谱及使用方便等特点而被广泛使用。对包括Wny-1和yn-6在内的81个玉米自交系进行烟嘧磺隆敏感性试验，并以Wny-1和yn-6为亲本进行烟嘧磺隆敏感性遗传研究。通过Wny-1和yn-6杂交、回交得到F1、BC1群体，进而分析烟嘧磺隆敏感性遗传规律；利用BC1群体中的烟嘧磺隆敏感单株对该敏感基因进行SSR标记定位。结果表明，81个玉米自交系中有8个敏感，73个耐烟嘧磺隆；yn-6对烟嘧磺隆的敏感性由1对隐性核基因控制，该基因位于第5染色体短臂上5S-96和5S-78标记之间，物理距离约为0．18Mb，暂时将该基因命名为nsfy。

关键词: 烟嘧磺隆, 敏感性, 遗传分析, 基因定位

Abstract: The sulfonylurea herbicide, nicosulfuron, has been used widely and rapidly for weed control, because its advantages such as low application rates,low toxicity, broad-spectrum and flexible application. A total of 81 corn inbreds including Wny-1 and yn-6 were evaluated for tolerance to nicosulfuron. F1, BC1 populations were constructed on the basis of the hybridization between Wny-1 and yn-6 and were used for genetic analysis of nicosulfuron sensi- tivity. Sensitive plants in BC1 population were used for mapping this gene with SSR markers. Of 81 inbreds evalua- ted,73 were resistant and 8 were susceptible. A single recessive nuclear gene which was located on the short arm of chromosome 5 determined sensitivity to nicosulfuron in yn-6. This gene was mapped between 5S-96 and 5S-78 with physical distance of 0.18 Mb and temporarily named nsfy.

Key words: Sulfonylurea, Sensitivity, Genetic analysis, Gene mapping

中图分类号:

S513.023

洪翠萍, 韩帅, 张彦军, 王立静, 魏海忠, 钟世宜, 刘保申. 玉米烟嘧磺隆敏感基因的遗传分析及基因定位[J]. 华北农学报, 2012, 27(4): 149-152. doi: 10.3969/j.issn.1000-7091.2012.04.028.

HONG Cui-ping, HAN Shuai, ZHANG Yan-jun, WANG Li-jing, WEI Hai-zhong, ZHONG Shi-yi, LIU Bao-shen. Genetic Analysis and Gene Mapping of Sensitivity to Nicosulfuron in Corn[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 149-152. doi: 10.3969/j.issn.1000-7091.2012.04.028.

http://www.hbnxb.net/CN/Y2012/V27/I4/149

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