华北农学报 ›› 2012, Vol. 27 ›› Issue (4): 149-152. doi: 10.3969/j.issn.1000-7091.2012.04.028

所属专题: 玉米 生物技术

• 论文 • 上一篇    下一篇

玉米烟嘧磺隆敏感基因的遗传分析及基因定位

洪翠萍, 韩帅, 张彦军, 王立静, 魏海忠, 钟世宜, 刘保申   

  1. 山东农业大学农学院, 山东泰安271018
  • 出版日期:2012-08-28
  • 通讯作者: 刘保申(1966-),男,山东武城人,教授,博士生导师.主要从事作物杂种优势与利用研究。
  • 作者简介:洪翠萍(1986-),女,山东滨州人,在读硕士,主要从事作物杂种优势与利用研究。
  • 基金资助:
    国家科技支撑计划项目(2011BAD35B01);山东省农业良种产业化项目(鲁农良种字[2011]7号)

Genetic Analysis and Gene Mapping of Sensitivity to Nicosulfuron in Corn

HONG Cui-ping, HAN Shuai, ZHANG Yan-jun, WANG Li-jing, WEI Hai-zhong, ZHONG Shi-yi, LIU Bao-shen   

  1. Agronomy College, Shandong Agricultural University, Tai'an 271018, China
  • Published:2012-08-28

摘要: 磺酰脲类除草剂烟嘧磺隆由于低用量、低毒、广谱及使用方便等特点而被广泛使用。对包括Wny-1和yn-6在内的81个玉米自交系进行烟嘧磺隆敏感性试验,并以Wny-1和yn-6为亲本进行烟嘧磺隆敏感性遗传研究。通过Wny-1和yn-6杂交、回交得到F1、BC1群体,进而分析烟嘧磺隆敏感性遗传规律;利用BC1群体中的烟嘧磺隆敏感单株对该敏感基因进行SSR标记定位。结果表明,81个玉米自交系中有8个敏感,73个耐烟嘧磺隆;yn-6对烟嘧磺隆的敏感性由1对隐性核基因控制,该基因位于第5染色体短臂上5S-96和5S-78标记之间,物理距离约为0.18Mb,暂时将该基因命名为nsfy。

关键词: 烟嘧磺隆, 敏感性, 遗传分析, 基因定位

Abstract: The sulfonylurea herbicide, nicosulfuron, has been used widely and rapidly for weed control, because its advantages such as low application rates,low toxicity, broad-spectrum and flexible application. A total of 81 corn inbreds including Wny-1 and yn-6 were evaluated for tolerance to nicosulfuron. F1, BC1 populations were constructed on the basis of the hybridization between Wny-1 and yn-6 and were used for genetic analysis of nicosulfuron sensi- tivity. Sensitive plants in BC1 population were used for mapping this gene with SSR markers. Of 81 inbreds evalua- ted,73 were resistant and 8 were susceptible. A single recessive nuclear gene which was located on the short arm of chromosome 5 determined sensitivity to nicosulfuron in yn-6. This gene was mapped between 5S-96 and 5S-78 with physical distance of 0.18 Mb and temporarily named nsfy.

Key words: Sulfonylurea, Sensitivity, Genetic analysis, Gene mapping

中图分类号: 

引用本文

洪翠萍, 韩帅, 张彦军, 王立静, 魏海忠, 钟世宜, 刘保申. 玉米烟嘧磺隆敏感基因的遗传分析及基因定位[J]. 华北农学报, 2012, 27(4): 149-152. doi: 10.3969/j.issn.1000-7091.2012.04.028.

HONG Cui-ping, HAN Shuai, ZHANG Yan-jun, WANG Li-jing, WEI Hai-zhong, ZHONG Shi-yi, LIU Bao-shen. Genetic Analysis and Gene Mapping of Sensitivity to Nicosulfuron in Corn[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 149-152. doi: 10.3969/j.issn.1000-7091.2012.04.028.

使用本文