小麦杂交F<sub>1</sub>和双亲幼苗动态蛋白质组分析

doi:10.3969/j.issn.1000-7091.2012.02.017

摘要/Abstract

摘要： 分别以2,3,5 d叶龄的栽培小麦京411、偏硬001和杂交F₁幼苗为材料,利用动态蛋白质组技术,对F₁及双亲的蛋白质变化做了比较分析,结果表明,在双亲及F₁中蛋白质差异表达现象被观察到,其中在5 d的叶子中最为明显。如在发育2 d和3 d的F₁叶子中,各有15个蛋白质斑点的含量产生变化,而在5 d叶龄的叶子中,这种有变化的蛋白质斑点达27个,在这些变化的蛋白质中,偏双亲和低于双亲的为多数,如5 d的叶子中分别占41%和44%,同时,在F₁叶片中观察到有蛋白质消失和新的蛋白质诱导现象产生。这些结果表明,杂种中存在蛋白质的差异表达现象,展开这方面的研究有助于对杂种优势形成机理的理解。

关键词: 杂种优势, 动态蛋白质组, 差异表达, 小麦, 偏双亲

Abstract: In this study，proteome approaches were applied to a comparative analysis of hybridization in wheat?F₁-hybrid and its parent lines by using 2，3，5 d leaves. The results indicated that protein expression differences were?observed between F₁-hybrid and parents. In the 5 d leaves，27 protein spots were changed in the protein abundances?and only 15 protein spots were changed in the protein abundances at 2 d，3 d leaves in F₁hybrid. In these changed?proteins，most protein spots were showed "two parental lines"characteration(41%) and "low parents"charactera?tion(44%) in protein abundances. we also observed the some protein sports were lost and some new protein spots?were induced in the F₁-hybrid. These results indicated that hybridization between two parental lines could cause ex?pression differences between wheat hybrid and its parents at protein abundances.

Key words: Hybridzation, Dynamic proteome, Difference expression, Wheat, Parental ditype parents

中图分类号:

S512.1
Q816

甘富, 段爽, 彭永康. 小麦杂交F₁和双亲幼苗动态蛋白质组分析[J]. 华北农学报, 2012, 27(2): 85-90. doi: 10.3969/j.issn.1000-7091.2012.02.017.

GAN Fu, DUAN Shuang, PENG Yong-kang. Dynamic Changes of Leaves Proteome Between Wheat F₁-hybrid and Its Parental Lines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 85-90. doi: 10.3969/j.issn.1000-7091.2012.02.017.

http://www.hbnxb.net/CN/Y2012/V27/I2/85

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小麦杂交F₁和双亲幼苗动态蛋白质组分析

Dynamic Changes of Leaves Proteome Between Wheat F₁-hybrid and Its Parental Lines

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小麦杂交F1和双亲幼苗动态蛋白质组分析

Dynamic Changes of Leaves Proteome Between Wheat F1-hybrid and Its Parental Lines

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小麦杂交F₁和双亲幼苗动态蛋白质组分析

Dynamic Changes of Leaves Proteome Between Wheat F₁-hybrid and Its Parental Lines