摘要： 为了进一步研究多种植酸酶之间的相互作用，利用PCR技术，从黑曲霉WP1中克隆出植酸酶PhyB基因，该基因长1560 bp，含有3段内含子，共编码460个氨基酸，与已报道的ALKO243的植酸酶Aph基因(GeneBank Acces-sion：L02420)相比较，编码的氨基酸序列同源性为99.13%，因此将其命名为PhyB₁。该基因含有植酸酶保守序列“RHGXRXP”和“HD”。黑曲霉WP1植酸酶PhyB₁基因序列已在国际基因库中注册，注册号为：DQ836360。

关键词: 黑曲霉, PhyB基因, PCR, 序列分析

Abstract: The PhyB₁ encoding phytase of Aspergillus niger WP1 was amplified by the polymerase Chain reaction (PCR) with primers designed according to the sequences in GeneBank. The amplified fragment was cloned and sequenced. The results showed that the coding region was 1560 bp in size, includes 3 introns, and encoded a peptide of 460 amino acid residues. Comparison of this sequence with the Aph of the nature A. niger ALKO243 (GeneBank Accession: L02420), shows that the nucleotide homology is 96.79%, and the amino acid homology comes up to 99.13%. The PhyB₁ ofA. niger WP1 strain in this paper its sequence has been accessed by GeneBank (Accession: DQ836360).

Key words: Aspergillus niger, PhyB gene, PCR, Sequence analysis

中图分类号: 

• Q789