华北农学报 ›› 2006, Vol. 21 ›› Issue (S1): 23-27. doi: 10.3321/j.issn:1000-7091.2006.z1.006

所属专题: 玉米

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东北地区主要玉米自交系的SSR遗传多样性分析

肖木辑1,2, 李明顺2, 李新海2, 张世煌2   

  1. 1. 沈阳农业大学, 辽宁 沈阳 110161;
    2. 中国农业科学院作物科学所玉米研究中心, 北京 100081
  • 收稿日期:2006-06-28 出版日期:2006-12-31
  • 作者简介:肖木辑(1965-),男,辽宁朝阳人,在读博士,高级农艺师,主要从事玉米育种研究工作;张世煌为通讯作者。
  • 基金资助:
    农业部“948”项目(2003-Q03);国家自然基金项目(30300223);AMBIONET项目资助

Genetic Diversity of Maize Inbred Lines Revealed by SSR in Northeast Provinces

XIAO Mu-ji1,2, LI Ming-shun2, LI Xin-hai2, ZHANG Shi-huang2   

  1. 1. Shenyang Agriculture University, Liaoning 110161, China;
    2. The Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2006-06-28 Published:2006-12-31

摘要: 利用70对扩增产物具有稳定多态性的SSR标记研究了66份玉米自交系的遗传多态性。70对引物在供试材料中共检测出273个等位基因变异,每对引物检测等位基因2~7个,平均3.9个,每个位点的多态性信息量(PIC)变化于0.121~0.814之间,平均0.584。66份自交系之间的遗传相似系数变化范围0.70~0.93。UPGMA聚类分析结果表明,66份供试自交系划分为5个类群,分类结果与系谱来源基本一致,东北地区生产上主要推广杂交种的亲本大多来自不同的类群。

关键词: SSR, 玉米自交系, 多态性信息量, 遗传多样性

Abstract: Simple sequence repeats (SSRs) were used to detect genetic diversity among 66 maize (Zea mays L.) inbred lines, which were widely used in northeast provinces of China. Seventy selected SSR primers gave stable profiles amplified in samples of 66 inbred lines. Seventy primers produced 273 polymorphic amplified fragments. The average number of allele per SSR locus was 3.9 which varied from 2 to 7. The polymorphism information content (PIC) for the SSR loci varied from 0.121 to 0.814 with an average of 0.584. Genetic similarities among the 66 inbred lines ranged from 0.62 to 0.93. The cluster analysis showed that the inbred lines could be classified into 5 distinct clusters. The results were consistent with the groups based on the available pedigree. Most of the parents used in maize commercial hybrids came from opposite clusters.

Key words: Simple sequence repeats, Maize inbred line, Polymorphism information content (PIC), Genetic diversity

引用本文

肖木辑, 李明顺, 李新海, 张世煌. 东北地区主要玉米自交系的SSR遗传多样性分析[J]. 华北农学报, 2006, 21(S1): 23-27. doi: 10.3321/j.issn:1000-7091.2006.z1.006.

XIAO Mu-ji, LI Ming-shun, LI Xin-hai, ZHANG Shi-huang. Genetic Diversity of Maize Inbred Lines Revealed by SSR in Northeast Provinces[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 23-27. doi: 10.3321/j.issn:1000-7091.2006.z1.006.

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