华北农学报 ›› 2005, Vol. 20 ›› Issue (4): 40-43. doi: 10.3321/j.issn:1000-7091.2005.04.011

所属专题: 白菜

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大白菜新型胞质雄性不育系及其保持系花药不同发育时期内源激素动态变化的研究

冯忠梅1, 张凤兰2, 张德双2, 于贤昌1   

  1. 1. 山东农业大学园艺学院, 山东泰安 271018;
    2. 北京市农林科学院蔬菜中心, 北京 100089
  • 收稿日期:2004-11-13 出版日期:2005-08-28
  • 通讯作者: 张凤兰
  • 作者简介:冯忠梅(1980- ),女,山东泰安人,硕士,主要从事白菜遗传等方面的研究工作。
  • 基金资助:
    北京市自然基金资助项目(6012008);国家863项目(2001AA241124,2002AA207012)

Study on Changes of Hormones in Anther at Different Development Stage in Cytoplasmic Male Sterility Line and Its Maintainer of Chinese Cabbage

FENG Zhong-mei1, ZHANG Feng-lan2, ZHANG De-shuang2, YU Xian-chang1   

  1. 1. Horticulture Department, Shandong Agricultural University, Tai'an 271018, China;
    2. Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China
  • Received:2004-11-13 Published:2005-08-28

摘要: 利用细胞质雄性不育系制种是生产白菜一代杂交种子最经济、有效的途径之一,植物内源激素在植物体内普遍存在,并在植物生长发育的各个阶段起着重要的调节作用。大量的研究结果表明,雄性不育系的营养器官或生殖器官中内源激素含量与保持系不同,雄性败育是花器官尤其是雄蕊中激素不平衡的结果。利用酶联免疫测定技术研究大白菜新型胞质雄性不育系及其保持系不同发育时期花药组织中IAA、GA3、ABA和ZR含量的动态变化,结果表明:在花药发育过程中,不育系花药组织中ABAI、AA、GA3和ZR含量及ZR/ABA比值的变化均出现异常。在第3时期(花蕾长2.0~3.0mm),不育系的IAA、GA3和ZR含量显著低于保持系,ABA含量显著高于保持系,ZR/ABA比值低于保持系。这一时期正好是不育系小孢子明显败育的时期。由此认为,该时期不育系花药组织中激素含量的变化及平衡的破坏可能影响了小孢子的正常发育,引起小孢子败育,导致大白菜的雄性不育。

关键词: 大白菜, 细胞质雄性不育系, 花药, 发育时期, 激素

Abstract: Changes of contents of IAA,ABA,GA3and ZR in anther at different development stage in Cytoplasmic Male Sterility (CMS)line and its maintainer of Chinese cabbage were investigated by ELISA (enzyme-linked immunosorbent assay).It is observed that changes of contents of IAA,ABA,GA3and ZR in anther of CytoplamicMale Sterility line were different to its maintainer.At the third stage (the length of flora bud was 2.0-3.0 mm),the contents of IAA,GA3and ZR incMS and the value of ZR/ABA were remarkably lower than its maintainer.However,the contents of ABA incMS were significantly higher than its maintainer.The microspores have just started to be sterile at this stage.It is concluded that the deficiency of IAA,GA3and ZR and over product of ABA and breaking of hormonal equilibrium induced male sterility in Chinese cabbage.

Key words: Chinese cabbage, Cytoplasmic male sterility, Anther, Development stage, Hormones

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引用本文

冯忠梅, 张凤兰, 张德双, 于贤昌. 大白菜新型胞质雄性不育系及其保持系花药不同发育时期内源激素动态变化的研究[J]. 华北农学报, 2005, 20(4): 40-43. doi: 10.3321/j.issn:1000-7091.2005.04.011.

FENG Zhong-mei, ZHANG Feng-lan, ZHANG De-shuang, YU Xian-chang. Study on Changes of Hormones in Anther at Different Development Stage in Cytoplasmic Male Sterility Line and Its Maintainer of Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(4): 40-43. doi: 10.3321/j.issn:1000-7091.2005.04.011.

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