摘要： 为探究香鳞毛蕨DfTCP基因应答外源激素和非生物胁迫的响应机制，以香鳞毛蕨幼苗为材料，在外源激素（SA、ABA、MeJA和Eth）、盐（200 mmol/L NaCl）、干旱（20% PEG）、高温（42℃）及低温（4℃）处理下，分别于0，0.5，1，3，6，12，24，48 h取样，对DfTCP进行生物信息学分析和表达模式分析。结果表明：DfTCP基因CDS全长1 431 bp，编码477个氨基酸，含有一个保守的bHLH结构域，定位于细胞外；DfTCP蛋白二级结构为mixed型；香鳞毛蕨不同组织中DfTCP的相对表达量为叶柄 > 叶 > 根；外源激素SA处理下，香鳞毛蕨叶片中DfTCP的相对表达量表现为“升-降-升-降”的趋势，在0.5，12 h达到峰值，极显著高于对照；ABA处理下，DfTCP的相对表达量整体上调且呈现先升后降的趋势，仅0.5 h香鳞毛蕨叶片中DfTCP的相对表达量与对照组差异不显著；MeJA和Eth处理后，香鳞毛蕨叶片中DfTCP的相对表达量均随处理时间的增长表现为“降-升-降”的趋势，分别在6，1 h达到峰值且与对照差异极显著；在PEG和NaCl条件下处理，香鳞毛蕨叶片中DfTCP的相对表达量均随处理时间的延长呈现“降-升-降”的变化规律，分别在6，12 h达到峰值且极显著高于对照；不同温度处理时，香鳞毛蕨叶片中DfTCP的相对表达量均呈先增后降的趋势，高温和低温分别在0.5，3 h达到峰值，极显著高于对照。综上，香鳞毛蕨叶片中DfTCP对不同外源激素响应的模式不同，且DfTCP在不同时间点响应不同的非生物胁迫，表明DfTCP在香鳞毛蕨抗逆方面发挥正向调节作用。

关键词: 香鳞毛蕨, DfTCP, 外源激素, 非生物胁迫, 生物信息学

Abstract: In order to explore the response mechanism of DfTCP gene of Dryopteris fragrans to exogenous hormones and abiotic stress, the seedlings of Dryopteris fragrans were treated with exogenous hormones (SA,ABA,MeJA and Eth),salt (200 mmol/L NaCl),drought (20% PEG),high temperature (42℃) and low temperature(4℃),samples were taken at 0,0.5,1,3,6,12,24,48 h respectively,bioinformatics analysis and expression pattern analysis were carried out for DfTCP. The results showed that the total length of CDS of DfTCP gene was 1 431 bp,which encoded 477 amino acids. The expressed product contained a conserved bHLH domain,which was located outside the cell;the secondary structure of DfTCP was mixed type;the relative expression of DfTCP in different tissues of Dryopteris fragrans was petiole > leaf > root;under the treatment of exogenous hormone SA,the relative expressions of DfTCP in the leaves of Dryopteris fragransshowed a trend of "ascending-descending-ascending-descending",they reached the peak value at 0.5,12 h,which were extremely significantly higher than the control;under ABA treatment,the relative expressions of DfTCP in the leaves of Dryopteris fragrans were up-regulated and showed a trend of increased first and then decreased,but there was no significant difference between 0.5 h and the control group;after MeJA and Eth treatment,the relative expressions of DfTCP in the leaves of Dryopteris fragransshowed a trend of "descending-ascending-descending" with the increase of treatment time,reached the peak value at 6,1 h respectively,and the difference was extremely significant compared with the control;under the condition of PEG and NaCl treatment,the relative expressions of DfTCP in the leaves of Dryopteris fragransshowed a law of "drop-rise-drop" with the extension of treatment time,and reached the peak value at 6,12 h respectively,which were extremely significantly higher than that of the control;the relative expressions of DfTCP in the leaves of Dryopteris fragrans increased first and then decreased at different temperatures,and reached the peak at 0.5,3 h respectively at high and low temperature,which were extremely significantly higher than that of the control. In summary,the response patterns of DfTCP in the leaves of Dryopteris fragrans to different exogenous hormones were different,and DfTCP responded to different abiotic stresses at different time points,indicating that DfTCP played a positive role in the stress resistance of Dryopteris fragrans.

Key words: Dryopteris fragrans, DfTCP, Exogenous hormones, Abiotic stress, Bioinformatics

中图分类号: 

• Q78