影响不结球白菜真空渗入转基因频率的几个因素

doi:10.3321/j.issn:1000-7091.2003.04.010

华北农学报 ›› 2003, Vol. 18 ›› Issue (4): 35-38. doi: 10.3321/j.issn:1000-7091.2003.04.010

所属专题： 白菜；生物技术；

影响不结球白菜真空渗入转基因频率的几个因素

曹传增¹, 刘凡², 赵泓², 蔡民华¹, 徐恒戬³

1. 首都师范大学, 生物系, 北京 100037;
2. 北京蔬菜研究中心, 北京 100089;
3. 山东农业大学, 园艺学院, 山东泰安 271018

收稿日期:2003-03-10 出版日期:2003-12-28
作者简介:曹传增(1977-),男,山东费县人,在读研究生,主要从事抗虫转基因方面的研究工作.

Several Factors Affecting the Vacuum Infiltration Gene Transformation in Non-heading Chinese Cabbage

CAO Chuan-zeng¹, LIU Fan², ZHAO Hong², CAI Min-hua¹, XU Heng-jian³

1. Department of Biology, Capital Normal University, Beijing100089, China;
2. Beijing Vegetable Research Center, Beijing100089, China;
3. Horticulture College, Shandong Agricultural University, Taian271000, China

Received:2003-03-10 Published:2003-12-28

摘要/Abstract

摘要： 采用不同发育阶段的植物材料及不同菌株,将马铃薯胰蛋白酶抑制剂基因(PinⅡ)和蜘蛛杀虫肽(SIP)基因双价转入9菜心,以除草剂抗性株出现频率,以及目的基因的PCR鉴定结果为依据,对影响不结球白菜-9菜心真空渗入转化率的一些因素进行了探索.结果表明植株生长阶段和真空渗入处理时间、所用菌株类型均对转化率有影响,其中以开花初期的植株,利用胭脂碱型农杆菌介导,真空渗入5 min的处理方法转化效率最高.

关键词: 真空渗入法, 双价抗虫基因, 不结球白菜

Abstract: Several factors affecting the vacuum infiltration transformation efficiency in Chinese cabbage (Brassica parachinensis Bailey) were studied.The developmental status of seedlings,vacuum infiltration time,type of Agrobacterium tumefaciens used,all influenced the transformation frequency.Via selection by herbicide Basta in the progeny and identification of the occurrence of the target gene by PCR, the transformation frequency was compared in different treatments.It was concluded that using the nopaline type of Agrobacterium,5 min vacuum infiltration,and using the plants with just several blooming flowers would give the best results.

Key words: Vacuum infiltration transformation, Insect-resistant genes, Non-heading Chinese cabbage

中图分类号:

曹传增, 刘凡, 赵泓, 蔡民华, 徐恒戬. 影响不结球白菜真空渗入转基因频率的几个因素[J]. 华北农学报, 2003, 18(4): 35-38. doi: 10.3321/j.issn:1000-7091.2003.04.010.

CAO Chuan-zeng, LIU Fan, ZHAO Hong, CAI Min-hua, XU Heng-jian. Several Factors Affecting the Vacuum Infiltration Gene Transformation in Non-heading Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(4): 35-38. doi: 10.3321/j.issn:1000-7091.2003.04.010.

http://www.hbnxb.net/CN/Y2003/V18/I4/35

参考文献

[1] Paszkowski J,Pisan B,Shillito R D,et al. Genetic transfor-mation of Brassica campestris var rapa protoplasts with an engineered Cauliflower Mosaic Virus genome[J].Plant Mol Biol,1986,6:303-312.
[2] Christey M C,Sinclair B K,Braun R H,et al.Regeneration of transgenic vegetable Brassicas (Brassica oleracea and B.campestris) via Ri-mediated transformation[J].Plant Cell Rep,1997,16:587-593.
[3] Dunwell J M.In vitro regeneration from excised leaf discs of three Brassica species[J].J Exp Bot,1981,32:789-799.
[4] Murata M,Orton T J.Callus initiation and regeneration capac-ities of three Brassica species[J].Plant Cell Triss Org Cult,1987,11:111-123.
[5] Feldmann Ka,Marks M D.Agrobacterium-mediated transform-ation of germinating seeds of Arabidopsis thaliana:a non-tissue culture approach[J].Mol Gen Genet,1987,208:1-9.
[6] Bechtold N,Ellis J,Pelletier G.In planta Agrobacterium med-iated gene transfer by infiltration of adult Arabidopsis thaliana plants C R [J].Acad Sci Paris Life Sci,1993,316:1194-1199.
[7] Liu F,Cao M Q,Yao L.In Planta Transformation of Pakchoi (Brassica Campestris L.SSP.Chinensis) by Infiltration of Adult Plants with Agrobacterium[J].Acta Hort,1998,467:187-192.
[8] 张广辉,巩振辉,薛万新,等.大白菜和油菜真空渗入遗传转化法初报[J].西北农业大学学报,1998,26(4):1-4.
[9] Anthony T Trieu,Stephen H,Burleigh,et al.Transformation of Medicago truncatula via infiltration seedlings or flowering plants with Agrobacterium[J].The Plant Journal,2000,22(6):531-541.
[10] Clark M S.植物分子生物学--实验手册[M].北京:高等教育出版社,施普林格出版社,1998.3-6.
[11] Ye G N,Stone D,Pang S Z,et al.Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation[J].The Plant Journal,1999,19(3):249-257.
[12] Clough S J,Bent A F.Floral di:a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana[J].The Plant Journal,1998,16(6):735-743.
[13] Christine Desfeux,Steven J Clough,Andrew F Bent.Female Reproductive Tissues Are the Primary Target of Agrobacterium-Mediated Transformation by the Arabidopsis Floral-Dip Method[J].Plant Physiology,2000,123:895-904.
[14] De Block M,De Brouwer D,Tenning P.Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants[J].Plant Physiol,1989,91: 694-701.
[15] Flavell R,Dart E,Fuchs R,et al.Selectable Marker Genes: Safe For Plants[J].Bio/Technology,1992,10:141-144.
[16] Tague B W.Germ-line transformation of Arabidopsis lasiocarpa[J].Transgenic Research,2001,10:259-267.
[17] Chang S,Park S K,Kim B C,et al.Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta[J].Plant Journal,1994,5(4):551-558.

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