摘要： 在ISTA小麦和大麦种子醇溶蛋白聚丙烯酰胺凝胶电泳鉴定品种的标准程序的基础上,对影响电泳效果的各主要因素进行逐一筛选、改进,尤其是实验设计的NaOH-醋酸-甘氨酸缓冲系统和先低后高再低的电泳方式使此项技术具有快速、分辨率较高、重复性较好、易剥胶、操作简单、经济实用等优点,适于一般实验室利用小麦种子醇溶蛋白技术对小麦进行大量的快速简捷的筛选、鉴定等初步研究.

关键词: 小麦, 种子醇溶蛋白, 凝胶电泳

Abstract: Based on the standard procedure of polyacrylamide geLelectrophotesis set by ISTA for the analysis of the gliadin of wheat and barley seed for the variety identification, we screened the main influence factors which would affect the electrophoresis, and tried to improve them to get the best combination.According to the results, we established an optimaLprocedure, it had the buffer system of Sodium Hydroxide Acetic Acid Glycine, and the voltage of electrophoresis should be lower at beginning, then higher for a while, and lower in the end.T his method had the properties of high resolution and stability, using less samples, and the geLcan be easily desqua-mated than the other methods.Furthermore, this method is easily to operate, more economical, and spend less time than the standard procedures, so it can be used in many laboratories to study the gliadin of wheat.

Key words: Wheat, Gliadin of seed, Polyacrylamide gel electrophotesis

中图分类号: 

• S512.01