摘要： 应用IBD快速检测试纸和琼脂凝胶免疫扩散试验(AGIDT),对不同疫苗免疫鸡检测结果均为阴性,对人工感染强毒鸡最早检出IBDV的时间,试纸检测法为感染后36 h,琼脂扩散检测法为感染后96 h.试纸检出的最低含毒量为800 ELD0,ELISA检出的最低含毒量为400 ELD0,琼扩检出的最低含毒量为2.×104 ELD0,试纸检出的敏感性比琼扩提高32～64倍.IBD试纸检测简便、快速、敏感、特异,是诊断鸡法氏囊病的有效方法.

关键词: IBD快速检测试纸, 检测, 传染性法氏囊病

Abstract: The results of the poultry flocks injected with a variety of IBD vaccines were negative by both IBD rapid diagnosis strip and agar gel immunodiffusion testing(AGIDT).The earliest times of detecting IBDV from the poultry flocks infected artif icially with IBDV were 36 and 96 hours af ter injecting for the IBD rapid diagnosis strip detecting and the AGIDT, respectively.The limits of detecting IBDV for the IBD rapid diagnosis strip, ELISA and AGIDT were 800 ELD50, 400 ELD50 and 2.5 ×10⁴ ELD50, respectively.The IBD rapid diag nosis strip was 32～64 times as sensitive as the AGIDT.The IBD rapid diagnosis strip detecting was a effective diagnosis method with simple, rapid, sensitive and specific advantages

Key words: IBD rapid diagnosis strip, Detecting, Infectious bursal disease

中图分类号: 

• S88.28.1+2