摘要： 利用产肠毒素大肠埃希氏菌(ETEC)标准菌株K88,K99,987P制备灭活苗,免疫待开产母鸡,应用琼脂扩散试验检测母鸡血清抗体和卵黄抗体(IgY)效价,在琼扩效价≥1∶64时收集卵黄,卵黄经氯仿去脂、硫酸胺沉淀后提纯出卵黄抗体,将提纯卵黄抗体对攻毒仔猪口服治疗,结果显示治疗仔猪腹泻状况明显减轻,与对照组相比有显著差异;治疗组未出现死亡而对照组仔猪有33%～67%的死亡率.试验结果表明,研制的IgY对ETEC所致的仔猪腹泻具有明显的效果.
关键词:
仔猪腹泻,
产肠毒素大肠埃希氏菌,
卵黄抗体
Abstract: Egg yolk immunoglobulins were achieved against K88, K99, 987P fimbrial adhesins of enterotox igenic Escherichia coli from egg yolk of chickens immunized with ETEC. The antibody activity was measured by ag argel precipitation assay. The egg yolk was gathered after its titer up to 1B64. The IgY was purified by chloroform ext raction and ammonium sulfate precipitation. With experimental infection, the neonatal piglets were treated orally with IgY solut ions after they showed symptoms of diarrhea. The mortality rate and the fecal scoring were used to evaluate the differences between the antibody treatment group and the control group. The experiment showed that the mortality rate was 33%-67% in the control group, whereas there was no death in the antibody treatment group. T he result indicated that egg yolk immunoglobulins can be used for curing pig let diarrhea.
Key words:
Pig let diarrhea,
ETEC,
IgY
中图分类号:
徐福洲, 王金洛, 杨兵, 宋维平, 赖平安, 秦泽荣, 孟彦妮. 抗猪产肠毒素大肠埃希氏菌卵黄抗体的研制及攻毒治疗试验[J]. 华北农学报, 2002, 17(4): 108-112. doi: 10.3321/j.issn:1000-7091.2002.04.024.
XU Fu-zhou, WANG Jin-luo, YANG Bing, SONG Wei-ping, LAI Ping-an, QIN Ze-rong, MENG Yan-ni. Preparation and Application of Chicken Egg Yolk Immunoglobulins Against Enterotoxigenic Escherichia coli in Piglets[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 108-112. doi: 10.3321/j.issn:1000-7091.2002.04.024.