簇毛麦和中间偃麦草rRNA基因位点双色荧光原位杂交分析

doi:10.3321/j.issn:1000-7091.2002.01.002

华北农学报 ›› 2002, Vol. 17 ›› Issue (1): 6-10. doi: 10.3321/j.issn:1000-7091.2002.01.002

所属专题： 生物技术；

簇毛麦和中间偃麦草rRNA基因位点双色荧光原位杂交分析

裴自友¹, 袁文业¹, 孙善澄¹, 孙玉¹, 富田因则², 安室喜正²

1. 山西省农业科学院作物遗传研究所, 山西太原 030031;
2. 鸟取大学农学部育种学研究室, 日本鸟取 680-8553

收稿日期:2001-04-28 出版日期:2002-03-28
作者简介:裴自友(1965-),男，副研究员,主要从事小麦染色体工程育种与分子细胞遗传学研究工作.
基金资助:
山西省农业科学院青年基金资助项目

Double Color FISH of rRNA Genes in Wheat Relatives of H.villosa and Th.ihtermedium

Pei Ziyou¹, Yuan Wenye¹, Sun Shancheng¹, Sun Yu¹, M Tomita², Y Yasumuro²

1. Crop Genetics Research Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;
2. Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan

Received:2001-04-28 Published:2002-03-28

摘要/Abstract

摘要： 簇毛麦和中间偃麦草是小麦改良的重要抗源,为了对导入的外源染色体及片段进行有效鉴定,应用分带和双色荧光原位杂交,将 25S-5,8S-18SrRNA、5SrRNA基因分别定于簇毛麦染色体1V短臂和5V短臂上。分别在中间偃麦草的3对、4对染色体上观察到 25S-5.8S-18S rRNA和 5SrRNA基因,其中有2对染色体在其短臂上有两种核糖体RNA基因。

关键词: 簇毛麦, 中间偃麦草, rRNA基因, 双色荧光原位杂交

Abstract: Haynaldia villosa(2n=14,VV)and Thinopyrum intermedium(2n=42,E₁E₁E₂E₂StSt)are important wheat relatives providing useful resistant genes for wheat improvement.In this paper, rRNA genes coding 25S-5. 8S-18S rRNA and SS rRNA are visualized on H.villosa and Th.intermedium chromosomes by double color fluorescence in situ hybridization. The 25S-5.8S-18S rRNA genes are localized on the short arm of 1V chromosome,and 5S rRNA genes are on the short arm of SV chromosome of H.villasa.On the other hand, three pairs of the 25S-5.8S-18S rRNA genes and four pairs of the SS rRNA genes are observed on the Th.intermedium chromosomes,in which 2 pairs of chromosomes have both rRNA genes on their short arms.

Key words: Haynaldia villosa, Thinopyrum intermedium, rRNA genes, Two color FISH

中图分类号:

Q943

裴自友, 袁文业, 孙善澄, 孙玉, 富田因则, 安室喜正. 簇毛麦和中间偃麦草rRNA基因位点双色荧光原位杂交分析[J]. 华北农学报, 2002, 17(1): 6-10. doi: 10.3321/j.issn:1000-7091.2002.01.002.

Pei Ziyou, Yuan Wenye, Sun Shancheng, Sun Yu, M Tomita, Y Yasumuro. Double Color FISH of rRNA Genes in Wheat Relatives of H.villosa and Th.ihtermedium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(1): 6-10. doi: 10.3321/j.issn:1000-7091.2002.01.002.

http://www.hbnxb.net/CN/Y2002/V17/I1/6

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簇毛麦和中间偃麦草rRNA基因位点双色荧光原位杂交分析

Double Color FISH of rRNA Genes in Wheat Relatives of H.villosa and Th.ihtermedium

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簇毛麦和中间偃麦草rRNA基因位点双色荧光原位杂交分析

Double Color FISH of rRNA Genes in Wheat Relatives of H.villosa and Th.ihtermedium

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