华北农学报

华北农学报 ›› 2002, Vol. 17 ›› Issue (1): 11-15. doi: 10.3321/j.issn:1000-7091.2002.01.003

所属专题： 生物技术；

• 论文 • 上一篇    下一篇

樱桃ACC氧化酶基因的克隆和序列分析

王俊英1, 张开春2, 王俊平3, 张军科4   

  1. 1. 北京林业大学生物学院, 北京 100083;
    2. 北京市农林科学院林果所, 北京 100093;
    3. 中国农业大学作物学院, 北京 100094;
    4. 西北农林科技大学园艺系, 陕西杨凌 712100
  • 收稿日期:2001-10-26 出版日期:2002-03-28
  • 作者简介:王俊英(1972-),女,北京林业大学在读博士,主要从事植物生物技术研究方面的工作。

Cloning and Sequencing of ACJC Oxidase Genes in Cherry Fruit and Leaf

Wang Junying1, Zhang Kaichun2, Wang Junping3, Zhang Junke4   

  1. 1. Beijing Forestry University, Beijing 100083, China;
    2. Beijing Agriculture and Forestry Institute, Beijing 100093, China;
    3. China Agricultural University, Beijing 100094, China;
    4. Northwestern Univwsity of Agric;ultural and Forestry Seience and Technology, Yangling, 712100, China
  • Received:2001-10-26 Published:2002-03-28

PDF

摘要： 以樱桃基因组DNA为模板定向克隆其ACC氧化酶基因。对PCR扩增的目的片段用 1%琼脂糖凝胶电泳检测后,进行回收、连续转化,通过蓝白筛选挑取白色菌落提取质粒DNA进行酶切鉴定,确定重组子后采用双脱氧终止法测定DNA全序列。DNA测序结果显示,樱桃的ACC氧化酶基因序列全长为 1310bp,由 2个外显子和 3个内含子构成,外显子总长为1000bp.同源性分析可知,樱桃与桃的ACC氧化酶基因DNA序列同源性达97.8%。

关键词: 樱桃, ACC氧化酶, 基因克隆, DNA测序

Abstract: The ACC oxidase gene was cloned from genomic DNA of prunus pseudocerasus L.Destination segments amplified by PCR were reclaimed,connected and transformed after exam nation by 1% agarose gel electrophoresis.By blue-white screening,the plasmid DNA extracted from white colony was identified using restriction enzyme analysis and the DNA sequence was determined following recon determination.The results of DNA sequencing revealed that the ACC oxidase gene sequence of Prunus pseudocerasus L was 1310 by and consisted of 2 exons and 3 introns and the full length of the exons was 1000 bp.Homogenous analysis indicated that homelogue of DNA sequence of the ACC oxidase gene in Prunus pseudocerasus L and Prunus persica Batsch reached 97.8%.

Key words: Cherry, ACC oxidase, Gene cloning, DNA sequencing

中图分类号: 

引用本文

王俊英, 张开春, 王俊平, 张军科. 樱桃ACC氧化酶基因的克隆和序列分析[J]. 华北农学报, 2002, 17(1): 11-15. doi: 10.3321/j.issn:1000-7091.2002.01.003.

Wang Junying, Zhang Kaichun, Wang Junping, Zhang Junke. Cloning and Sequencing of ACJC Oxidase Genes in Cherry Fruit and Leaf[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(1): 11-15. doi: 10.3321/j.issn:1000-7091.2002.01.003.

使用本文

0
    /   /   推荐 /   导出引用

链接本文: http://www.hbnxb.net/CN/10.3321/j.issn:1000-7091.2002.01.003

               http://www.hbnxb.net/CN/Y2002/V17/I1/11

版权所有 © 2022 《华北农学报》编辑部

地址：石家庄市和平西路598号 邮政编码：050051

电话： 0311-87652166 邮箱：hbnxb@163.com

本系统由北京玛格泰克科技发展有限公司设计开发 冀ICP备09020210号-1