摘要： 采用温室苗期模拟NaCl盐分胁迫技术,对来自12个国家的13份中间偃麦草种质材料叶片的相对含水量、细胞质膜透性、游离脯氨酸和丙二醛含量等生理特征的影响进行了综合评价,结果显示,随着NaCl胁迫时间的延长和浓度的增加,相对含水量呈下降趋势,细胞质膜透性、游离脯氨酸和丙二醛含量则呈上升趋势。对上述4个生理指标进行相关性与综合聚类分析,将13份中间偃麦草种质划分为3个耐盐级别:即耐盐种质有EI037、EI038、EI043,中度耐盐种质有EI017、EI031、EI044、EI046、EI049,敏盐种质有EI020、EI030、EI041、EI042、EI045。综合NaCl胁迫下的盐害级别和存活率情况,初步认为0.9%是中间偃麦草种质苗期可耐受NaCl胁迫的浓度阈值。
关键词:
中间偃麦草,
苗期,
NaCl胁迫,
生理响应
Abstract: The NaCl stress simulating technology at seedling stage in greenhouse was used for the studies on the ef- fects of salinity and days treatments on physiological characteristics,such as the relative water content(RWC),cell mem- brance permeability(CMP),content of free proline (Pro) and Malondaildehyde (MDA)of 13 germplasm and materials of E.intermedia (Host)Nevski collecting from 12 different countries.The results showed that the RWC was decreased,but the CMP,Pro and MDA were increased when the stress of salinity and days were intensified.According to the correlation and cluster analysis of 4 physiological indexes,13 germplasm and materials of E.intermedia were divided into 3 levels of salt tolerance,e.g. the strong salt tolerance of EI037,EI038,EI043,the weak EI020,EI030,EI041,EI042,EI045,and the medium EI017,EI031,EI044,EI046,EI049.Furthermore,0.9 %of NaCl concentration was considered as the salinity threshold of E.intermedia germplasm at seedling stage based on above 4 physiological indexes,the salinity tolerance rat- ings and livability.
Key words:
Elytrigia intermedia (Host)Nevski,
Seedling stage,
NaCl stress,
Physiological responses
中图分类号:
尚春艳, 张国芳, 毛培春, 孟林, 安沙舟. 中间偃麦草苗期NaCl胁迫的生理响应[J]. 华北农学报, 2008, 23(4): 157-162. doi: 10.7668/hbnxb.2008.04.036.
SHANG Chun-yan, ZHANG Guo-fang, MAO Pei-chun, MENG Lin, AN Sha-zhou. Responses of Physiological Characteristics of E. intermedia at Seedling Stage under NaCl Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 157-162. doi: 10.7668/hbnxb.2008.04.036.