摘要： 通过Gus基因在谷子胚性愈伤组织中的瞬时表达检测，分析了影响谷子愈伤组织基因枪转化效率的几个因素。钨粉的包被质粒DNA用量以3μg/mg钨粉，CaCl₂浓度1.5mol/L，亚精胺浓度40mmol/L为最佳，过高的DNA、CaCl₂和亚精胺加入量均显着降低转化率；JQ 700基因枪样品室高度7cm，轰击速度400~450m/s转化，较其它速度和样品室高度下转化Gus基因表达率高；每次转化的愈伤组织用量对转化效率也有影响，以1~2gGus基因表达斑最多；玉米ubiquitin启动子较CaMV35S启动子的瞬时表达显着增强。

关键词: 谷子, 基因枪, 瞬时表达, 参数优化

Abstract: Factors influencing foxtail millet (Setaria italica Beauv. ) embryogenic calli transformation by particle bombardments were studied using the method of Gus transient expression with JQ-700 gene gun. Relatively high numbes of Gus transient expression unites were obtained when the quantity of plasmid DNA added to microprojectles was 4 μg/mg tungsten powder and the concentration of CaCl₂ and spermidine used to absorb DNA to microprojectles were 1.5 mol and 40 mmol respectively. The velocity of the microprojectles and the distance between the calli being bombarded and the stopping plate of JQ-700 gene gun optimized in this report were 400-450 m/s and 7cm. The quantity of calli used per treatment was also a factor influencing gene delivery and 1-2g of calli per treatment gave higher Gus transient expression. The promoter derived from maize ubiquitin produced significantly more Gus foci compared to the CaMV35s Promoter.

Key words: Foxtail millet embryogenic calli, Particle bombardment, Gus transient expression, Parameter optimizing