摘要： 用高效液相色谱（HPLC）法测定了31个南瓜品种果肉中β-胡萝卜素的含量。取干粉样品用石油醚提取，旋转蒸干，正已烷定容，然后进行HPLC测定。分析柱为Nova-PakC18柱，流动相为90%甲醇与乙酸乙酯洗脱梯度，在450nm处检测。测得品种间β-胡萝卜素的含量有显着差异，西班牙南瓜中β-胡萝卜素的含量8.735mg/100g（鲜重）,远远高于其它南瓜品种，甚至高于胡萝卜中β-胡萝卜素的含量，为富含β-胡萝卜素的高保健南瓜。

关键词: 南瓜, &beta, －胡萝卜素, 反相高效液相色谱

Abstract: β-carotene from pulps of 31 varieties of pumpkin were determined by reversed phase high performance liquid chromatography （RP HPLC） with ultraviolet （UV） detector（254nm）,a C18 column and an eluant of 90% methanol and ethyl acetate.Dried powder samples were extracted by petroleum ether,evaporated to dryness and then dissolved by hexane to a final ratio of 1g of dried sample per 10ml hexane.The hexane solutions were analysed by HPLC.Difference of contents of β carotene between pumpkin varieties were found significant.Content of β carotene in spanish pumpkin was 8 735mg/100g .F.W.,which was far higher than that of other pumpkin varieties and even higher than the content of β carotene in carrot.Spanish pumpkin can be considered as a β carotene rich vegetable.

Key words: &beta, carotene, Pumpkin, RP HPLC

中图分类号: 

• S642.1