绿菜花游离小孢子培养、胚胎发生和植株再生

doi:10.3321/j.issn:1000-7091.1998.03.020

摘要/Abstract

摘要： 取花蕾长3.0～5.5mm的花序，7%（W/V）饱和次氯酸钙溶液（加一滴吐温20）表面消毒15min,无菌水洗3次。然后轻压花蕾，过滤，收集滤液，离心后，将小孢子悬浮于经过滤灭菌的改良的1/2NLN培养基中。石蜡膜封口，置温度梯度培养箱作32.5℃、1d的高温热激诱导处理，后置25℃下继续培养。本试验对13份绿菜花供试材料的游离小孢子培养能力进行初步研究，其中8种基因型（占全部材料的61.5%）有胚胎发生，并获得5种基因型的再生株，共207株。基因型对绿菜花游离小孢子培养的影响很大，基因型“巴绿青花菜”出胚率最高，达37.08胚/蕾。同时，对培养基中加入活性炭悬浮液和更换培养基的作用进行了研究。

关键词: 绿菜花, 游离小孢子培养, 胚胎发生, 植株再生

Abstract: Buds of 3 0～5.5mm in length of broccoli (Brassica oleracea var.italica) were removed from the raceme,surface sterilized with full strength commercial bleach (7% sodium hypochlorite)for 15 min and rinsed 3 times with sterile deionized water.Then they were squeezed,filtered,prepared and centrifuged.The density of the suspension was cultured in modified liquid medium 1/2 NLN lacking growth substances.They were sealed with parafilm and incubated in dardness at 32.5℃ for one day and then incubated at 25℃ continually.13 genotypes of broccoli were used in the isolated microspore culture study.Microspore derived embryos were produced in 8 genotypes and 207 microspore derived plants were produced in 5 genotypes. Of the eight genotypes in which microspore derived embryos were gained,the best one was"Balu broccoli"which produced 37.08 embryos per bud.Genotypes influenced isolated microspore culture of broccoli significantly.The addition of active carbon suspension to culture media and the alternation of culture media were also studied.

Key words: Broccoli, Isolated microspore culture, Embryogenesis, Plant regeneration

中图分类号:

S635.303

张德双，曹鸣庆，秦智伟. 绿菜花游离小孢子培养、胚胎发生和植株再生[J]. 华北农学报, 1998, 13(3): 102-106. doi: 10.3321/j.issn:1000-7091.1998.03.020.

Zhang Deshuang， Cao Mingqing. Embryogenesis and Plant Regeneration of Broccoli via Isolated Microspore Culture[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(3): 102-106. doi: 10.3321/j.issn:1000-7091.1998.03.020.

http://www.hbnxb.net/CN/Y1998/V13/I3/102

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