摘要： 17个用于大白菜常规育种的基因型在NLN-13液体培养基中进行游离小孢子培养，结果有16个基因型经由小孢子胚胎发生途径获得小孢子胚。这些基因型包括从早熟到晚熟的各种类型。各基因型之间在小孢子胚胎发生频率方面存在很大差异，产量最高的两个基因型T11和CC11，平均每百花蕾分别达35928和34132个胚。供体植株生长环境对小孢子胚胎发生具有明显影响：在10/20℃（夜/昼）的部分控温温室生长的供体植株，其小孢子胚产量较高；而在温度无法调控、午间最高温达30-40℃、夜间降至10-15℃左右田间生长的供体植株，胚产量仅为前者的29.5%。

关键词: 游离小孢子培养, 胚胎发生, 基因型, 供体植株, 生长温度, 大白菜

Abstract: Of 17 Chinese cabbage (Brassica campestris ssp. pekinensis) genotypes, 16 genotypes had produced microspore-derived embryos via isolated microspore culture. The production of microspore-derived embryos varied remarkably between these genotypes. The two genotypes with the highest embryo yield, T11 and CC11, produced 35928 and 34132 embryos per 100 flower buds, respectively. The growth environment (temperature) of donor plants obviously affected microspore embryogenesis. Average embryo yield of donor plants of CC11 grown in the greenhouse at 10℃/20℃ ( night/day) was 12 991.6 embryos per 100flower buds. But that of CC11 grown in the field at 10-15 ℃/0-40℃ (night/day)was 3,831.5, or 29.5% as high as the former.

Key words: Isolated microspore culture, Embryogenesis, Genotype, Donor plant, Growth temperature, Chinese cabbage (Brassica campestris ssp. pekinensis)