华北农学报 ›› 1993, Vol. 8 ›› Issue (1): 65-69. doi: 10.3321/j.issn:1000-7091.1993.01.013

所属专题: 小麦

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小麦胚和盾片中过氧化物酶同工酶与品种抗感白粉病的关系

王立新, 古旭, 胡道分   

  1. 北京市植物细胞工程实验室, 北京 100081
  • 收稿日期:1992-01-13 出版日期:1993-03-28

Relationship between Peroxidase Isozymes in Embryos plus Scutella of Wheat and Resistance/Susceptibility to Powdery Mildew in Wheat

Wang Lixin, Gu Xu, Hu Daofen   

  1. Beijing Plant Cell Bioengineering Laboratory, Beijing 100081
  • Received:1992-01-13 Published:1993-03-28

摘要: 对40个小麦、4个黑麦和2个小黑麦品种及品系的胚和盾片进行同工酶电泳分析,发现抗病和感病材料的过氧化物酶酶谱的主要差异在酶谱中部(B区)。B区中一级酶带多,且在具有Rf0.50、0.56两条一级酶带的材料中,86%表现为抗白粉病。B区中一级酶带少的或缺少0.50、0.56带的材料中,91%感白粉病。因此认为用胚和盾片的过氧化物酶同工酶鉴定小麦对白粉病的抗性既简便易行,又具有较高的可靠性。

关键词: 小麦, 过氧化物酶同工酶, 白粉病

Abstract: Peroxidase isozymes electrophoretic analyses on embryos plus scutella from forty wheat varieties, four ryes and two triticales revealed differences in the middle of the zymograms (B range) of resistant and susceptible varieties.86% of the varieties with many more intense bands (MIB) including Rf 0.50 and Rf 0.56 bands at the B range showed resistance, while 91% of the varieties with few MIB's or with no Rf 0.50 and Rf 0.56 bands at the B range showed susceptibility.Therefore, peroxidase isozymes analyses on embryos plus scutella of wheat was considered to be a simple, practical and highly reliable method for the evaluation of resistance to powdery mildew of wheat.

Key words: Wheat, Peroxidase isozymes, Powdery mildew

引用本文

王立新, 古旭, 胡道分. 小麦胚和盾片中过氧化物酶同工酶与品种抗感白粉病的关系[J]. 华北农学报, 1993, 8(1): 65-69. doi: 10.3321/j.issn:1000-7091.1993.01.013.

Wang Lixin, Gu Xu, Hu Daofen. Relationship between Peroxidase Isozymes in Embryos plus Scutella of Wheat and Resistance/Susceptibility to Powdery Mildew in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(1): 65-69. doi: 10.3321/j.issn:1000-7091.1993.01.013.

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