摘要： 本文收集国内不同地区的谷子品种120个, 以及狗尾草、谷莠子和御谷等材料, 采用聚丙烯酰胺凝胶电泳法进行了酯酶同工酶分析。谷子芽期的酯酶同工酶酶带共出现19条, 所得酶谱分成31个类型。从地理分布来分析, 黄土高原区和内蒙古高原区品种酯酶同工酶类型丰富。从谷子品种的起源和演变来分析, 谷子和普通狗尾草的酶谱大致相同。研究表明, 酯酶同工酶重复性好, 比较稳定, 可以做为谷子分类学和研究谷子进化关系的一个生化指标。

关键词: 谷子, 凝胶电泳法, 酯酶同工酶

Abstract: 120 foxtail millet varieties(Setaria italica)and other related species were collected from various parts of China. Their esterase isoenzymes were analysed with polyacrylamide gel electrophoresis. During germinating period esterase isoenzymes of foxtail millet showed 19 enzyme bands and the zymograms obtained were classified as 31 types. Analysed from the view of geographic distribution, esterase isoenzymes of foxtail millet varieties on Loess plateau and Neimenggu plateau had abundant types, and analysed from the view of the origin and evolution of foxtail millet, foxtail millet had similar zymograms as green bristlegrass(S. viridis), The study showed that esterase isoenzymes were stable and had good repeatability. They could be used as a biochemical index for foxtail millet taxonomy and the study of its evolution.

Key words: Foxtail millet, Polyacrylamide gel electrophoresis, Esterase isoenzymes