In Yangtze river basin,little SSR primers and little genetic information is available for DNA fingerprints and diversity of short season cotton germplasms introduced from the northern of China,so,in present research,fifteen short season cotton introduced from Institute of Cotton Research,Chinese Academy of Agricultural Sciences(ICR,CAAS)were used as materials in construction of DNA fingerprinting and analysis of genetic diversity with simple sequence repeat(SSR)makers.Sixty-eight primer pairs with good repeatability,high polymorphism,almost even distribution on the 26 chromosomes were screened out from 214 pairs of candidate SSR primers to genotype the 15 materials,178 effective allelic loci were detected with an average of 2.62;Primers polymorphism information content(PIC)value ranged from 0.244 9 to 0.906 1 with an average of 0.693 8.Variety fingerprint analysis results showed that 12 cultivars could be differentiated by specific or characteristic primer,the rest 3 cultivars could be identified by primer combinations.In the end,four pairs of primers combinations to be detected in 21 polymorphic bands with an average of 5.25 could completely differentiate the 15 short season cotton cultivars from each other.NTSYS-pc2.1 software was used to analyze genetic diversity of the 15 cultivars,the results indicated that genetic similarity coefficient of the 15 cultivars ranged from 0.45 to 0.79,with an average of 0.62,the 15 materials can be divided into five major categories at the similarity coefficient of 0.68.combined with the pedigree of clustering to be carried on the system analysis,which provided basis for the short season cotton identification and parental material selection.
CHEN Hao-dong
,
XIAO Cai-sheng
,
LI Xiang
,
KUANG Zheng-cheng
,
WANG Wei
,
LI Yu-qiang
. DNA Fingerprints Construction and Genetic Diversity Analysis of Short-season Cotton by SSR Markers[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(4)
: 98
-104
.
DOI: 10.7668/hbnxb.2014.04.016
[1] Tatineni V,Cantrell R G,Davis D D.Genetic diversity in elite cotton germplasm determined by morphological characteristics and RAPDs[J].Crop Science,1996,36:186-192.
[2] 于霁雯.短季棉遗传多样性分析及其重要农艺和经济性状的QTL定位[D].武汉:华中农业大学,2006.
[3] 王心宇,郭旺珍,张天真,等.我国短季棉品种的RAPD指纹图谱分析[J].作物学报,1997,23(6):669-676.
[4] 张玉翠,杨伟华,匡猛,等.32个棉花主栽品种DNA指纹图谱构建及遗传多样性分析[J].棉花学报,2012,24(2):120-126.
[5] 韩宗福,王景会,申贵芳,等.黄河流域棉花主要品种SSR指纹图谱构建及遗传差异分析[J].棉花学报,2011,23(6):545-551.
[6] 李成奇,王晓芸,张晓飞,等.百棉系列棉花自交系品种(系)SSR指纹图谱构建[J].棉花学报,2011,23(3):228-234.
[7] 孙宁,杨付新,付小琼,等.国家棉花区试新品种的SSR指纹图谱分析[J].棉花学报,2011,23(3):279-283.
[8] 武文,邓启云,周丽洁,等.利用SSR 分子标记构建Y58S及部分重要两系杂交水稻亲本的DNA指纹图谱[J].杂交水稻,2008,23(3):52-56.
[9] 张建华,张金渝,杨晓洪,等.用SSR 标记建立玉米黄早四DNA标准指纹图谱的方法研究[J].西南农业学报,2006,19(3):345-350.
[10] 王通强,马晓峰,吴有祥.油菜杂种及亲本指纹图谱构建和杂种纯度鉴定[J].食品与生物技术学报,2009,28(3):377-384.
[11] 段艳凤,刘杰,卞春松,等.中国 88 个马铃薯审定品种 SSR 指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457.
[12] 李育强,陈浩东,洪亚辉,等.湘杂棉SSR 指纹图谱的构建及应用[J].棉花学报,2009,21(3):175-178.
[13] 匡猛,杨伟华,许红霞,等.中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析[J].中国农业科学,2011,44(1):20-27.
[14] 郭旺珍,张天真,潘家驹,等.我国棉花主栽品种的RAPD 指纹图谱研究[J].农业生物技术学报,1996,4(2):129-134.
[15] 陈浩东,刘方,王为,等.棉花多重PCR 技术及其对杂交棉纯度鉴定的初步研究[J].棉花学报,2011,23(1):22-27.
[16] 张艳欣,林忠旭,李武,等.海岛棉EST-SSR 引物的开发与应用研究[J].科学通报,2007,52(15):1779-1787.
[17] Chen G,Du X M.Genetic diversity of source germplasm of upland cotton in China as determined by SSR marker analysis[J].Acta Genetica Sinica,2006,33(8):733-745.
[18] 周忠丽,杜雄明,潘兆娥,等.亚洲棉种质资源的SSR遗传多样性分析[J].棉花学报,2013,25(3):217-226.
[19] 李成奇,王晓芸,刘莹,等.百棉3号、百棉018杂交种及其亲本SSR指纹图谱构建[J].河南农业科学,2011,40(6):56-59.
[20] 韩宗福,赵逢涛,王景会,等.鲁棉研系列抗虫棉品种SSR指纹图谱构建及遗传多样性分析[J].华北农学报,2013,28(增刊):1-6.
[21] 孙东磊,孙君灵,杜雄明,等.棕色棉与绿色棉遗传多样性比较研究[J].华北农学报,2009,24(5):96-101.
[22] 武耀廷,张天真,殷剑美.利用分子标记和形态学性状检测的陆地棉栽培品种遗传多样性[J].遗传学报,2001,28(11):1040-1050.
[23] 喻树迅,黄祯茂.短季棉品种早熟性构成因素的遗传分析[J].中国农业科学,1990,23(6):48-54.
[24] 陈仲方,张治伟,王支凤.陆地棉品种早熟性研究[J].江苏农业学报,1989,5(3):12-19.
[25] 薛艳,张新宇,沙红,等.新疆早熟棉品种SSR指纹图谱构建与品种鉴别[J].棉花学报,2010,(22)4:360-366.
[26] 姚全胜,詹儒林,黄丽芳,等.11个芒果品种SSR 指纹图谱的构建与品种鉴别[J].热带作物学报,2009,30(11):1572-1576.
