Acta Agriculturae Boreali-Sinica

Acta Agriculturae Boreali-Sinica >

2014 , Vol. 29 >Issue 4: 37 - 43

DOI: https://doi.org/10.7668/hbnxb.2014.04.007

Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl(t) in Rice

  • CHEN Tao ,
  • LIU Yan-qing ,
  • ZHANG Ya-dong ,
  • ZHU Zhen ,
  • ZHAO Qing-yong ,
  • ZHOU Li-hui ,
  • YAO Shu ,
  • YU Xin ,
  • ZHAO Ling ,
  • WANG Cai-lin
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  • Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China

Received date: 2014-04-19

  Online published: 2014-09-20

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Abstract

Leaf morphology is one of key components for rice plant architecture,so excavation,mapping and cloning related mutant genes are very important for revealing mechanism of leaf development and breeding new rice varieties with ideotype and super-high yield.In this study,the narrow and rolled mutant ntl(t) was found from the improved line of rice stripe disease for Wuyunjing No.7 and the mutant phenotype genetic analysis and gene mapping was researched.Compared with wild type,the mutant showed multipe abnormal phenotypes,such as shorter leaf length and width,rolled leaf,dwarfism,more tillers,reduced panicle length,branch number,filled grain number per spike,spikelets per spike,seed-setting rate and 1000-grain weight.Genetic analysis indicated that the mutant was controlled by a single recessive gene.Using 500 recessive mutant individuals in the F2 population derived from the cross between Fuhui 838 and nrl(t),the gene NRL (t) was located to a confined region of 160 kb flanked by two microsatellite markers RM11-01 and RM11-11 on the short arm of chromosome 11.Based on the annotation result of rice genome,26 open reading frames(ORFs)were predicted in this region.Sequencing analysis showed that the homeobox3A gene reported might be the potential candidate gene.These results are very valuable for further study on this gene.

Key words： Oryza sativa L.; Narrow and rolled leaf; Mutant; Genetic analysis; Gene mapping

Cite this article

CHEN Tao , LIU Yan-qing , ZHANG Ya-dong , ZHU Zhen , ZHAO Qing-yong , ZHOU Li-hui , YAO Shu , YU Xin , ZHAO Ling , WANG Cai-lin . Genetic Analysis and Gene Mapping of a Narrow and Rolled Mutant nrl(t) in Rice[J]. Acta Agriculturae Boreali-Sinica, 2014 , 29(4) : 37 -43 . DOI: 10.7668/hbnxb.2014.04.007

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