Analysis of Screening Wheat Drought Resistance Varieties by IRAP

  • LI Wei-tao ,
  • CHEN Qiu-fang ,
  • YA Hui-yuan ,
  • GU Yun-hong
Expand
  • 1. Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, China;
    2. Institution of Life Science, Luoyang Normal University, Luoyang 471022, China

Received date: 2014-03-26

  Online published: 2014-09-19

Abstract

Using IRAP molecular method, design primers and 36 wheat varieties were used for PCR amplification, polymorphic finger maps were obtained and clustering analysis.The results were as follows:246 bands were produced by IRAP, the range of amplification fragments were 250-2 000 bp, a total of 13 polymorphic loci, the range of different varieties was 0-12.the results of clustering analysis can be clearly differentiate drought resistance between 36 wheat varieties.The study showed that IRAP were suitable for screening drought resistance varieties in wheat.

Cite this article

LI Wei-tao , CHEN Qiu-fang , YA Hui-yuan , GU Yun-hong . Analysis of Screening Wheat Drought Resistance Varieties by IRAP[J]. Acta Agriculturae Boreali-Sinica, 2014 , 29(3) : 105 -108 . DOI: 10.7668/hbnxb.2014.03.020

References

[1] 郭凤芝,刘凤洲,郭凌云,等.小麦抗旱性品种筛选与分析[J].山东农业科学,2013,45(1):46-48.
[2] 魏添梅,昌小平,闵东红,等.小麦抗旱品种的遗传多样性分析及株高优异等位变异挖掘[J].作物学报,2010,36(6):895-904.
[3] 敬礼恒,刘利成,梅 坤,等.水稻抗旱性能鉴定方法及评价指标研究进展[J].中国农学通报,2013,29(12):1-5.
[4] 杨 琳,景继海,赵佰图.旱地小麦抗旱性鉴定指标研究[J]现代农业科技,2009(17):19-20.
[5] 赵会君,张怀刚,王海庆.抗旱性不同的春小麦品种籽粒萌发期α-淀粉酶活性及其同工酶分析[J].麦类作物学报,2008,28(4):633-637.
[6] 张文英,柳斌辉,彭海城,等.小麦品种抗旱性鉴定指标遗传规律研究[J].华北农学报,2008,23(增刊):92-95.
[7] 贾寿山,朱俊刚,王曙光,等.山西小麦地方品种萌发期的抗旱性[J].华北农学报,2011,26(2):213-217.
[8] 张立生,温辉芹,程天灵,等.小麦高产抗旱育种实践[J].山西农业科学,2011,39(12):1243-1246.
[9] Kalendar R, Grob T,Regina M,et al.IRAP and REMAP:two new retrotransposon-based DNA finger-printing techniques[J].Theor Appl Genet,1999,98:704-711.
[10] Kumar A,Bennetzen J I.Plant retrotransposons[J].Annu Rev Genet,1999,33:479-532.
[11] Leigh F,Kalendar R,Lea V,et al.Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques[J].Mol Genet Genom,2003,269(4):464-474.
[12] Petr Smykal.Development of an efficient retrotransposon-based fingerprinting method for rapid pea variety identification[J].J Appl Genetic,2006,47(3):221-230.
[13] Breto M P,Ruiz C,Pina JA.The diversification of Citrus clementina Hort.ex Tan.,a vegetatively propagated crop species[J].Mol Phylogenet Evol,2001,21(2):285-293.
[14] Gao L,McCarthy E M,Ganko E W,et al.Evolutionary history of Oryza sativa LTR retrotransposons:a preliminary survey of the rice genome sequences [J].BMC Genomics,2004,5(1):18.
[15] 侯清松,车京玉,邵立刚,等.小麦抗旱性遗传与研究进展[J].小麦研究,2013,34(2):6-13.
[16] Kristin A,Schneider,Mary E B,et al.Marker assisted selection to improve drought resistance in common Bean[J].Crop Sci,1997,37:51-60.
Outlines

/