In order to improve the regeneration frequency of somatic embryos of wheat immature embryo,the effects of different concentration AgNO3 on someclone culture of immature embryo callus and oxalate oxidase activities of immature embryogenic callus were studied in this paper from three wheat cultivars (Yumai 18,Yumai 49 and Lankao 906) which were cultured with more areas in Henan province.The result indicated that the differences of induction frequency of emryogenic callus andgreen spot rates of callus and oxalate oxidase activities of immature embryogenic callus were significant at 0.01 level among different AgNO3concentrations or genotypes.It was highest in Yumai 18 and was lowest in Lankao 906 among different genotypes.It were highest in MS medium suppliedwith 2.55.0 mg/ L AgNO3 among different AgNO3 concentrations.The coefficient between induction frequency and oxalate oxidase activities of immature emryogenic callus in wheat under different AgNO3concentrations was0.920 and it was significant at 0.01 level.So,the regeneration frequency can be increased in someclone culture of wheat immature embryo supplied with Ag+.
HE Sheng-lian
,
LEI Zhen-sheng
,
WU Zheng-qing
,
ZHAO Xian-lin
,
FANG Bao-ting
,
YANG Hui-min
,
WANG Mei-fang
,
HE Ning
,
YANG Pan
,
LI Wei
. Application of AgNO3 to Someclone Culture of Immature Embryo in Wheat[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(6)
: 198
-201
.
DOI: 10.7668/hbnxb.2009.06.040
[1] 王亚琴,梁承邺.硝酸银在水稻农杆菌转化中的作用[J].湖南大学学报:自然科学版,2004,31(2):28-31.
[2] 高武军,孙富丛,魏开发,等.玉米胚性愈伤组织诱导与分化的影响因素[J].华北农学报,2005,20(1):27-30.
[3] Purhauser L,Madgyesy M,Czeko P J,et al.Stimulation of shoot regeneration in Triticum aestivnm and Nicotiana ptumbaginifolia Viv.tissue culture using the ethylene inhibitor of AgNO_3[J].Plant Cell Reperts,1987,6:1-4.
[4] Meijer E G M,Simmonds J.Polyamine levels in relation to growth and somatic embryogenesis of Medicago sativa[J].J Ex Bot,1988,203:787.
[5] Torrigiani P,Altamura M M,Pasqua G,et al.Free and con-jugated polyamines during de novo floral vegetative bud for-mation in thin cell layers of tobacco[J].Plyhsiol Plant,1987,70:453.
[6] 崔凯荣,陈克明,王晓哲,等.植物体细胞胚胎发生研究的某些现状[J].植物学通报,1993,10(3):14.
[7] Mahmut Caliskan,Muge Turet,Andrew C Cuming.Formation of wheat embryogenic callus involves peroxide-generating germin-like oxalate oxidase[J].Planta,2004,219:132-140.
[8] Lane B G,Dunwell J M,Ray J A,et al.Germin,a protein marker of early plant growth,is an oxalate oxidase[J].Biochemistry Journal,1993,268:12239-12242.
[9] Lane B G,Andrew C,Cuming.Germin isoforms are discrete temporal markers of wheat development,Pseudogermin is a uniquely thermostable water-soluble oligomeric protein in ungerminated embryos and like germin in germinated embryos,it is incorporated in cell wall[J].European Journal of Biochemistry,1992,209:961-969.
[10] Lane B G.Oxalate,germin,and the extracellular matrix of higher plants[J].FASEB Journal,1994,8:294-301.
[11] 王常云,王作全,李晓亮.等.小麦幼胚离体培养育种技术研究[J].麦类作物学报,1999,19(1):14-16.
[12] 廖祥儒,张会图.甘露醇和AgNO_3对小麦细胞再分化的影响[J].河北大学学报:自然科学版,2000,20(3):263-265.
[13] Mahmut Caliskan,Andrew C Cuming.Spatial specificity of H_2O_2-generating oxalate oxidase gene expression during wheat embryo germination[J].The Plant Journal,1998,15(2):165-171.
