In order to study function of Ammopiptanthus mongolicus dehydrin gene,First,the primers were designed according to the sequence of Ammopiptanthus mongolicus dehydrin cDNA library,the dehydrin gene was amplified with PCR,then carried out site-directed mutagenesis for its a base internal,and cloned into pCAMBIA3301 vector.The dehydrin gene was introduced into sugar beet by Agrobacterium mediated tumerfaciens,and resistance to Km of Sugar beet were obtained,which lays a good foundation for the further research.
LI Xiao-dong1,2,BAI Chen1,ZHANG YU-jin2,YU Zhuo1,SUN Guo-qin2,ZHANG Hui-zhong2,GUO Jiu-feng2,KANG Lian-he2
. The Studies on Constructing Expression Vector of Ammopiptanthus mongolicus Dehydrin Gene and Genetic Transformation to Sugar Beet[J]. Acta Agriculturae Boreali-Sinica, 2010
, 25(1)
: 68
-74
.
DOI: 10.7668/hbnxb.2010.01.013
[1] 王忠华,李旭晨,夏英武.作物抗旱的作用机制及其基因工程改良研究进展[J].生物技术通报,2002(1):16-19.
[2] 张林生,赵文明.LEA蛋白与植物的抗旱性[J].植物生理学通讯,2003,39(1):61-66.
[3] 翟大勇,沈黎明.脱水蛋白研究进展明[J].生物化学与生物物理学进展,1998,25(2):119-122.
[4] 孙歆,雷韬,袁澍,等.脱水素研究进展[J].武汉植物学研究,2005,23(3):299-304.
[5] 郭九峰,孙国琴,沈传进,等.沙冬青cDNA文库的构建和EST分析[J].华北农学报,2007,22(4):37-41.
[6] Baker J,Steele C,Dure L.Sequence and characterization of 6 LEA proteins and their genes from cotton[J].Plant MolBiol,1988,ll:277-29.
[7] Close T J.ehydrins:emergence of a biochemical role of a family of plant dehydration proteins[J].Physl Plant,1996,97:795-803.
[8] 刘广宇,魏夸波,陈吉龙,等.植物脱水素研究进展[J].生物工程进展,2001,21(2):24-27.
[9] 黄发平,张林生,王路平,等.小麦脱水素基因WZY2的克隆及其序列分析[J].西北农林科技大学学报:自然科学版,2009,2(37):93-99.
[10] Allagulova C R,Gimalov F R,Shakirova F M,et al.The plant dehydrins:structure and putative functions[J].Biochemistry,2003,68:945 -951.
[11] 王君丹,胡莺雷,魏晓,等.脱水素基因转化的矮牵牛对干旱胁迫的反应[J].分子植物育种,2004,2(3):369-374.
[12] Masakazu H,Shogo T,Tomoko F,et al.Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco[J].Planta,2003,217:290-298.
[13] Hara M,Terashima S,Fukaya T,et al.Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco[J].Planta,2003,217:290-298.