Flavonoids of Sophora flavescens were extracted with solvent extraction on mycelial growth of Fusarium oxysporum f.sp.cucumerinum,disease resisting effect and resistance physiology with different concentrations were studied.The results showed that all the concentrations(0.25,0.5,1,2,4 g/L) of flavonoids of Sophora flavescens restrained the mycelial growth of Fusarium oxysporum f.sp.cucumerinum,and the stimulative rates were enhanced with the increased concentration.As the concentration of flavonoids was 4 g/L,the inhibition rate was 93.08%.In the therapeutic trail,plants have the biggest promtion by 60 g/L flavonoidswhich results indicated that chlorophyll content,root activity,superoxide dismutase(SOD) activity,peroxidase(POD) activity and phenylalanine ammonia-lyase(PAL) activity were increased 17.79%,71.55%,48.20%,123.80%,151.48 %comparing with control.Malondialdehyde(MDA) content and relative conductivity declined 62.33%and 71.14%comparing with control.The results of the prevention trail indicated that flavonoids of Sophora flavescens enhanced the disease resistance of cucumber at all the concentrations,and the best effect present in 80 g/L.
ZHOU Bao-li
,
YAO Ting
,
ZHANG Jian
,
YE Xue-ling
. Effects of Flavonoids of Sophora flavescens to Fusarium Wilt and Resistance Physiology of Cucumber[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(6)
: 78
-82
.
DOI: 10.7668/hbnxb.2009.06.016
[1] 王玲平.黄瓜感染枯萎病菌后生理生化变化及其与抗病性关系的研究[D].太谷:山西农业大学,2001.
[2] 刘邮洲,陈志谊,刘永锋.南京地区蔬菜枯萎病菌拮抗细菌的筛选与评价[J].江苏农业学报,2004,20(1):18-22.
[3] 刘保才,王俊琪,孙国语.蔬菜病虫害化学防治中的3R问题与科学使用农药[J].上海蔬菜,2004(6):68-69.
[4] Mohamed S,Saka S,El-sharksey S H,et al.Antimycotic screening of 58 Malaysian plants against plant pathogens[J].Pestic Science,1996,47:259-264.
[5] 吴凤芝,梦立君,文景芝.黄瓜根系分泌物对枯萎病菌菌丝生长的影响[J].中国蔬菜,2002(5):83-84.
[6] Wang W Q,Ben-daniel B H,Cohen Y.Extracts of Inula viscose control downy mildew caused by Plasmopara viticola in Grapebines[J].Phytoparasitica,2004,32:208.
[7] 陆志科,谢碧霞.不同种竹叶的化学成分及其提取物抗菌活性的研究[J].西北林业学院学报,2005,20(1):49-52.
[8] 王文桥,刘金朵,张文渊,等.植物抗生活性及植物源农药研究进展[J].河北农业科学,2005,9(4):74-79.
[9] 杨玉萍,李艾莲.植物源杀菌剂研究进展[J].中国农业科技导报,2006,8(1):49-53.
[10] 曲波,翟强.植物源农药的应用前景[J].辽宁农业科学,2003(3):36-36.
[11] 吴恭谦.三种毛茛科植物提取物及原白头翁素的活性研究[J].安徽农学院学报,1989(1):21-31.
[12] 王树桐.对番茄灰霉病菌有抗菌活性的植物提取物的室内筛选[J].河北农业大学学报,2003,26(1):61-64.
[13] 张淑红,周宝利,张磊,等.天然植物提取物对茄子黄萎病菌的抑制活性[J].应用生态学报,2006,17(6):1137-1140.
[14] 郑永权,姚建仁,邵向东,等.苦参杀虫抑菌生物活性的初步研究[J].植物保护,1999,25(6):17-19.
[15] Masae Y,Mikkiko K,Shuzo T.The effects of flavonoids on Staphylococcus aureus[J].Phytotherapy Research,1990,4(6):235-236.
[16] 郑玉艳,周宝利,张淑红,等.不同浓度苦参提取物对蔬菜病原菌的抑菌活性[J].植物保护,2007,33(5):121-123.
[17] 司红丽,王建华,王越虎.狗舌草总黄酮的提取及其毒性试验[J].畜牧与兽医,2003,35(7):9-10.
[18] 郝建军,刘延吉.植物生理学实验技术[M].沈阳:辽宁科学技术出版社,2001:162-166.
[19] 刘萍,李明军.植物生理学实验技术[M].北京:科学出版社,2007:123-125.
[20] 张志安,陈展宇.植物生理学实验技术[M].吉林大学出版社,2008:202-203.
[21] 段泽敏,王贤萍,周柏玲,等.苹果树腐烂病无公害防治技术研究[J].山西农业科学,2002,30(4):55-57.
[22] 郑玉艳,周宝利,张淑红.苦参黄酮对辣椒枯萎病的抑制效应[J].沈阳农业大学学报,2008,39(3):297-300.
[23] 董新纯,赵世杰,张元湖,等.类黄酮在草甘膦诱导的苦荞膜脂过氧化中的作用[J].植物保护学报,2006,33(4):412-416.
[24] 张树生,胡蕾,刘忠良,等.植物体内抗病相关酶与植物抗病性的关系[J].安徽农学通报,2006,12(13):48-49.
[25] 齐亚娥,吴冬青,安红钢,等.玫瑰花蕾黄酮类化合物提取及清除羟自由基能力[J].食品科学,2007,32(1):82-84.
[26] 魏国强,钱琼秋,朱祝军.黄瓜白粉病抗性及生理机制的研究[J].华北农学报,2004,19(2):84-86.
[27] 张鹏.银杏叶黄酮的微波提取及其抗氧化性研究[J].安徽农业科学,2009,37(12):5496-5497.
[28] 徐建华,王建波,利容千.黄瓜感染枯萎病后病理组织学的研究[J].植物病理学报,1997,27(4):349-352.